Anti-PI 3 Kinase p85 alpha Antibody [ARC55272] (A307709)

Name: Anti-PI 3 Kinase p85 alpha Antibody [ARC55272]
Brand: Antibodies.com
SKU: A307709
Price: 470.0 USD
Availability: InStock

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$470

Rabbit monoclonal [ARC55272] antibody to PI 3 Kinase p85 alpha for WB and IHC.

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Delivery

Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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Telephone

+1 (314) 370-6046

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Specifications

Name

Anti-PI 3 Kinase p85 alpha Antibody [ARC55272]

Description

Rabbit monoclonal [ARC55272] antibody to PI 3 Kinase p85 alpha.

Applications

WB, IHC

Dilutions

WB: 1:500-1:2,000, IHC: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 1-100 of human PI3 Kinase p85 alpha (P27986).

Sequence

MSAEGYQYRALYDYKKEREEDIDLHLGDILTVNKGSLVALGFSDGQEARPEEIGWLNGYNETTGERGDFPGTYVEYIGRKKISPPTPKPRPPRPLPVAPG

Host

Rabbit

Clonality

Monoclonal

Clone ID

ARC55272

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

85 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (3)

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Figure 1: Western Blot - Anti-PI 3 Kinase p85 alpha Antibody [ARC55272] (A307709)

Western blot analysis of extracts of various cell lines, using Anti-PI 3 Kinase p85 alpha Antibody [ARC55272] (A307709) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 3min.

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Figure 2: Immunohistochemistry - Anti-PI 3 Kinase p85 alpha Antibody [ARC55272] (A307709)

Immunohistochemistry analysis of paraffin-embedded mouse brain using Anti-PI 3 Kinase p85 alpha Antibody [ARC55272] (A307709) at a dilution of 1:60 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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Figure 3: Immunohistochemistry - Anti-PI 3 Kinase p85 alpha Antibody [ARC55272] (A307709)

Immunohistochemistry analysis of paraffin-embedded rat brain using Anti-PI 3 Kinase p85 alpha Antibody [ARC55272] (A307709) at a dilution of 1:60 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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