Anti-PI 3 Kinase p85 beta Antibody (A87727)

Name: Anti-PI 3 Kinase p85 beta Antibody
Brand: Antibodies.com
SKU: A87727
Price: 335.0 USD
Availability: InStock

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$335

Rabbit polyclonal antibody to PI 3 Kinase p85 beta for WB, IHC and ICC/IF.

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Delivery

Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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Telephone

+1 (314) 370-6046

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Specifications

Name

Anti-PI 3 Kinase p85 beta Antibody

Description

Rabbit polyclonal antibody to PI 3 Kinase p85 beta.

Applications

WB, IHC, ICC/IF

Dilutions

WB: 1:500-1:1,000, IHC: 1:50-1:200, ICC/IF: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-85 of human PI3 Kinase p85 beta (NP_005018.1).

Sequence

MAGPEGFQYRALYPFRRERPEDLELLPGDVLVVSRAALQALGVAEGGERCPQSVGWMPGLNERTRQRGDFPGTYVEFLGPVALAR

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

85 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.01% Thiomersal.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

p85, p85 beta, P85B, P85B_HUMAN, Phosphatidylinositol 3 kinase, Phosphatidylinositol 3 kinase regulatory beta subunit, Phosphatidylinositol 3 kinase regulatory subunit beta, Phosphatidylinositol 3 kinase regulatory subunit polypeptide 2, Phosphatidylinositol 3 kinase, regulatory subunit, polypeptide 2 (p85 beta), Phosphatidylinositol 3-kinase 85 kDa regulatory subunit beta, Phosphatidylinositol 3-kinase regulatory subunit beta, Phosphoinositide 3 kinase regulatory subunit 2, Phosphoinositide 3 kinase regulatory subunit 2 (beta), Phosphoinositide 3 kinase regulatory subunit polypeptide 2, Phosphoinositide 3 kinase regulatory subunit polypeptide 2 (p85 beta), Phosphoinositide 3 kinase, regulatory subunit 2 (beta), Phosphoinositide 3 kinase, regulatory subunit 2 (p85 beta), PI3 kinase p85 beta subunit, PI3 kinase p85 subunit beta, PI3-kinase regulatory subunit beta, PI3-kinase subunit p85-beta, PI3K, PI3K regulatory subunit beta, PIK3R 2, PIK3R2, PtdIns 3 kinase p85 beta, PtdIns-3-kinase regulatory subunit beta, PtdIns-3-kinase regulatory subunit p85-beta

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (5)

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Figure 1: Western Blot - Anti-PI 3 Kinase p85 beta Antibody (A87727)

Western blot analysis of extracts of various cell lines, using Anti-PI 3 Kinase p85 beta Antibody (A87727) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 90s.

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Figure 2: Immunohistochemistry - Anti-PI 3 Kinase p85 beta Antibody (A87727)

Immunohistochemistry analysis of paraffin-embedded rat ovary using Anti-PI 3 Kinase p85 beta Antibody (A87727) at a dilution of 1:200 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

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Figure 3: Immunohistochemistry - Anti-PI 3 Kinase p85 beta Antibody (A87727)

Immunohistochemistry analysis of paraffin-embedded human colon tissue using Anti-PI 3 Kinase p85 beta Antibody (A87727) at a dilution of 1:200 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

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Figure 4: Immunohistochemistry - Anti-PI 3 Kinase p85 beta Antibody (A87727)

Immunohistochemistry analysis of paraffin-embedded human placenta using Anti-PI 3 Kinase p85 beta Antibody (A87727) at a dilution of 1:200 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.