This antibody reacts with PRKAR2A (protein kinase A regulatory type II alpha subunit), previously identified under the general name "intra-acrosomal protein".
Applications
ICC, WB
Dilutions
IHC: 10 µg/ml; membrane permeabilization (acetone) is essential.
Reactivity
Human
Immunogen
Freshly ejaculated human sperms were washed in PBS and extracted in 3% acetic acid, 10% glycerol, 30 mM benzaminidine. The acid extract was dialyzed against 0.2% acetic acid and subsequently used for immunization.
Host
Mouse
Clonality
Monoclonal
Clone ID
Hs-36
Isotype
IgM
Conjugate
Unconjugated
Purification
Purified by differential precipitation and solid-phase chromatography.
Concentration
1 mg/ml
Product Form
Liquid
Formulation
Supplied in Tris Buffered Saline, pH 8, with 15 mM Sodium Azide.
