This antibody strongly reacts with human PKAc alpha subunit and weakly reacts with PKAc gamma subunit; both ~40 kDa. The recognized epitope of PKAc alpha is identical between human, sheep, porcine, ox, and dog.
Applications
WB
Dilutions
WB: 1 µg/ml; Positive Control: HeLa human cervix carcinoma cell line. To detect PKAc gamma, use a more concentrated lysate from a tissue expressing this subunit (testis).
Reactivity
Human
Immunogen
Synthetic peptide corresponding to sequence ESPAQNTAHLDQFERIK from human Protein Kinase A catalytic alpha (PKAc alpha).
Sequence
ESPAQNTAHLDQFERIK
Host
Mouse
Clonality
Monoclonal
Clone ID
6D2.1
Isotype
IgG1
Conjugate
Unconjugated
Purification
Protein A chromatography.
Concentration
1 mg/ml
Predicted MW
40 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.4, with 15 mM Sodium Azide.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.
Figure 1: Dot Blot - Anti-PKA C alpha Antibody [6D2.1] (A85978)
Dot Blot analysis of GST and GST-fusion proteins using Anti-PKAc Antibody (A85978) and Anti-GST Antibody [S-tag-05]. The total amount of material spotted on the nitrocellulose membrane is 5 ng/spot. Lane 1: GST-Akt1. Lane 2: GST-Akt2. Lane 3: GST-Akt3. Lane 4: GST-PKAc alpha. Lane 5: GST-PKAc beta. Lane 6: GST-PKAc gamma. Lane 7: GST-MEK 1. Lane 8: GST.
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