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Anti-PMCA1 Antibody (A308310)

Western Blot - Anti-PMCA1 Antibody (A308310) - Antibodies.com Immunofluorescence - Anti-PMCA1 Antibody (A308310) - Antibodies.com Immunofluorescence - Anti-PMCA1 Antibody (A308310) - Antibodies.com
$335
Product Datasheet

Rabbit polyclonal antibody to PMCA1 for WB and ICC/IF.

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$40 to United States
Lead Time
5-8 Business Days

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Name
Anti-PMCA1 Antibody
Description
Rabbit polyclonal antibody to PMCA1.
Applications
WB, ICC/IF
Dilutions
WB: 1:500-1:2,000, ICC/IF: 1:50-1:200
Reactivity
Human, Mouse, Rat
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 50-150 of human ATP2B1 (NP_001673.2).
Sequence
QESYGDVYGICTKLKTSPNEGLSGNPADLERREAVFGKNFIPPKKPKTFLQLVWEALQDVTLIILEIAAIVSLGLSFYQPPEGDNALCGEVSVGEEEGEGE
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugate

Unconjugated

Purification
Affinity purification.
Molecular Weight
140 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.05% Proclin 300.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Synonyms
AT2B1_HUMAN, ATP2B1, ATPase Ca++ transporting plasma membrane 1, Plasma membrane calcium ATPase 1, Plasma membrane calcium ATPase isoform 1, Plasma membrane calcium pump isoform 1, Plasma membrane calcium transporting ATPase 1, Plasma membrane calcium-transporting ATPase 1, PMCA 1
Isotype Controls
Suitable Secondaries
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
Western Blot - Anti-PMCA1 Antibody (A308310) - Antibodies.comEnlarge Image
Figure 1: Western Blot - Anti-PMCA1 Antibody (A308310)
Western blot analysis of extracts of Mouse brain, using Anti-PMCA1 Antibody (A308310) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 5s.
Immunofluorescence - Anti-PMCA1 Antibody (A308310) - Antibodies.comEnlarge Image
Figure 2: Immunofluorescence - Anti-PMCA1 Antibody (A308310)
Immunofluorescence analysis of mouse brain cells using Anti-PMCA1 Antibody (A308310) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).
Immunofluorescence - Anti-PMCA1 Antibody (A308310) - Antibodies.comEnlarge Image
Figure 3: Immunofluorescence - Anti-PMCA1 Antibody (A308310)
Immunofluorescence analysis of rat brain cells using Anti-PMCA1 Antibody (A308310) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).
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