Anti-PMS2 Antibody [ARC2479] (A309444)

Name: Anti-PMS2 Antibody [ARC2479]
Brand: Antibodies.com
SKU: A309444
Price: 470.0 USD
Availability: InStock

Enlarge Image

$470

Rabbit monoclonal [ARC2479] antibody to PMS2 for WB and IHC.

100% Guarantee

Price Match Guarantee

Delivery

Freight Charges

$40 to United States

Lead Time

5-8 Business Days

Contact Us

Telephone

+1 (314) 370-6046

orders@antibodies.com

Guarantees

100% Quality Guarantee

Price Match Guarantee

Tags:

Knockout Validated Antibody

Specifications

Name

Anti-PMS2 Antibody [ARC2479]

Description

Rabbit monoclonal [ARC2479] antibody to PMS2.

Applications

WB, IHC

Dilutions

WB: 1:500-1:1,000, IHC: 1:50-1:200

Reactivity

Human

Immunogen

Recombinant protein of human PMS2.

Host

Rabbit

Clonality

Monoclonal

Clone ID

ARC2479

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.05% Proclin 300.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

DNA mismatch repair gene homologue, DNA mismatch repair protein PMS2, HNPCC4, H_DJ0042M02.9, Mismatch repair endonuclease PMS2, Mismatch repair gene PMSL2, MLH4, PMS 2, PMS1 homolog 2 mismatch repair system, PMS1 protein homolog 2, PMS2 postmeiotic segregation increased 2, PMS2 postmeiotic segregation increased 2 (S. cerevisiae), PMS2CL, PMS2_HUMAN, PMSL2, Postmeiotic segregation increased, S. cerevisiae, 2

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (7)

Enlarge Image

Figure 1: Western Blot - Anti-PMS2 Antibody [ARC2479] (A309444)

Western blot analysis of extracts of Jurkat cells, using Anti-PMS2 Antibody [ARC2479] (A309444) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 10s.

Enlarge Image

Figure 2: Western Blot - Anti-PMS2 Antibody [ARC2479] (A309444)

Western blot analysis of extracts from wild type (WT) and PMS2 knockout (KO) HeLa cells, using Anti-PMS2 Antibody [ARC2479] (A309444) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 10s.

Enlarge Image

Figure 3: Immunohistochemistry - Anti-PMS2 Antibody [ARC2479] (A309444)

Immunohistochemistry analysis of paraffin-embedded human appendix tissue using Anti-PMS2 Antibody [ARC2479] (A309444) at a dilution of 1:200 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Enlarge Image

Figure 4: Immunohistochemistry - Anti-PMS2 Antibody [ARC2479] (A309444)

Immunohistochemistry analysis of paraffin-embedded human colon cancer loss of PMS2 expression (negative control samples) using Anti-PMS2 Antibody [ARC2479] (A309444) at a dilution of 1:200 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.