Anti-PNMT Antibody [ARC2786] (A305507)

Name: Anti-PNMT Antibody [ARC2786]
Brand: Antibodies.com
SKU: A305507

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This product is discontinued

Anti-PNMT Antibody [ARC2786] (A305507) has been discontinued and is no longer available.

View all Anti-PNMT Antibodies.

Specifications

Name

Anti-PNMT Antibody [ARC2786]

Description

Rabbit monoclonal [ARC2786] antibody to PNMT.

Applications

WB, ICC/IF

Dilutions

WB: 1:500-1:1,000, ICC/IF: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 183-282 of human PNMT (P11086).

Sequence

CLEAVSPDLASFQRALDHITTLLRPGGHLLLIGALEESWYLAGEARLTVVPVSEEEVREALVRSGYKVRDLRTYIMPAHLQTGVDDVKGVFFAWAQKVGL

Host

Rabbit

Clonality

Monoclonal

Clone ID

ARC2786

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

31 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

Noradrenaline N-methyltransferase, PENT, Phenylethanolamine N-methyltransferase, PNMTase, PNMT_HUMAN

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (3)

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Figure 1: Western Blot - Anti-PNMT Antibody [ARC2786] (A305507)

Western blot analysis of extracts of various cell lines, using Anti-PNMT Antibody [ARC2786] (A305507) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Enhanced Kit (RM00021). Exposure time: 60s.

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Figure 2: Western Blot - Anti-PNMT Antibody [ARC2786] (A305507)

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Figure 3: Immunofluorescence - Anti-PNMT Antibody [ARC2786] (A305507)

Immunofluorescence analysis of human adrenal gland cells using Anti-PNMT Antibody [ARC2786] (A305507) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).

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