Anti-POLD3 Antibody (A10090)

Name: Anti-POLD3 Antibody
Brand: Antibodies.com
SKU: A10090
Price: 335.0 USD
Availability: InStock

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$335

Rabbit polyclonal antibody to POLD3 for WB, IHC and ICC/IF.

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Delivery

Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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Telephone

+1 (314) 370-6046

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Specifications

Name

Anti-POLD3 Antibody

Description

Rabbit polyclonal antibody to POLD3.

Applications

WB, IHC, ICC/IF

Dilutions

WB: 1:500-1:2,000, ICC/IF: 1:50-1:100, IHC: 1:100-1:500

Reactivity

Human, Mouse

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 197-466 of human POLD3 (NP_006582.1).

Sequence

ASKAAAKTQETNKETKTEAKEVTNASAAGNKAPGKGNMMSNFFGKAAMNKFKVNLDSEQAVKEEKIVEQPTVSVTEPKLATPAGLKKSSKKAEPVKVLQKEKKRGKRVALSDDETKETENMRKKRRRIKLPESDSSEDEVFPDSPGAYEAESPSPPPPPSPPLEPVPKTEPEPPSVKSSSGENKRKRKRVLKSKTYLDGEGCIVTEKVYESESCTDSEEELNMKTSSVHRPPAMTVKKEPREERKGPKKGTAALGKANRQVSITGFFQRK

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

51 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

DNA polymerase delta subunit 3, DNA polymerase delta subunit p66, DNA polymerase delta, subunit 3, DNA polymerase subunit delta p66, DPOD3_HUMAN, KIAA0039, P66, P68, Pol delta C subunit (p66), Polymerase (DNA directed), delta 3, Polymerase (DNA-directed), delta 3, accessory subunit, PPP1R128, Protein phosphatase 1, regulatory subunit 128

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (4)

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Figure 1: Western Blot - Anti-POLD3 Antibody (A10090)

Western blot analysis of extracts of various cell lines, using Anti-POLD3 Antibody (A10090) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Enhanced Kit (RM00021). Exposure time: 60s.

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Figure 2: Immunohistochemistry - Anti-POLD3 Antibody (A10090)

Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue using Anti-POLD3 Antibody (A10090) at a dilution of 1:300 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

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Figure 3: Immunofluorescence - Anti-POLD3 Antibody (A10090)

Immunofluorescence analysis of GFP-RNF168 transgenic U2OS cells using Anti-POLD3 Antibody (A10090). Green:GFP-RNF168 fusion protein expression for DNA damage marker. DAPI was used to stain the cell nuclei (blue). RNF168(GFP) can be used to mark cells damaged by UV-A laser for they always gather around DNA damage region.