Anti-PON1 Antibody [ARC2001] (A308894)

Name: Anti-PON1 Antibody [ARC2001]
Brand: Antibodies.com
SKU: A308894
Price: 470.0 USD
Availability: InStock

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$470

Rabbit monoclonal [ARC2001] antibody to PON1 for WB, IHC and ICC/IF.

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Delivery

Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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+1 (314) 370-6046

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Specifications

Name

Anti-PON1 Antibody [ARC2001]

Description

Rabbit monoclonal [ARC2001] antibody to PON1.

Applications

WB, IHC, ICC/IF

Dilutions

WB: 1:500-1:2,000, IHC: 1:50-1:200, ICC/IF: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 50-150 of human PON1 (P27169).

Sequence

TGSEDLEILPNGLAFISSGLKYPGIKSFNPNSPGKILLMDLNEEDPTVLELGITGSKFDVSSFNPHGISTFTDEDNAMYLLVVNHPDAKSTVELFKFQEEE

Host

Rabbit

Clonality

Monoclonal

Clone ID

ARC2001

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

40 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

A esterase 1, A-esterase 1, Aromatic esterase 1, Arylesterase 1, Arylesterase B type, ESA, Esterase A, K 45, K-45, MVCD5, Paraoxonase, Paraoxonase 1, Paraoxonase B type, Paraoxonase, plasma, Paraoxonase1, PON, PON 1, PON1_HUMAN, Serum aryldiakylphosphatase, Serum aryldialkylphosphatase 1, Serum paraoxonase/arylesterase 1

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (6)

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Figure 1: Western Blot - Anti-PON1 Antibody [ARC2001] (A308894)

Western blot analysis of extracts of Mouse liver, using Anti-PON1 Antibody [ARC2001] (A308894) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 1s.

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Figure 2: Western Blot - Anti-PON1 Antibody [ARC2001] (A308894)

Western blot analysis of extracts of HepG2 cells, using Anti-PON1 Antibody [ARC2001] (A308894) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 3s.

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Figure 3: Immunohistochemistry - Anti-PON1 Antibody [ARC2001] (A308894)

Immunohistochemistry analysis of paraffin-embedded human liver using Anti-PON1 Antibody [ARC2001] (A308894) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

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Figure 4: Immunofluorescence - Anti-PON1 Antibody [ARC2001] (A308894)

Immunofluorescence analysis of rat liver using Anti-PON1 Antibody [ARC2001] (A308894) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).