Anti-PP1C gamma Antibody (A14509)

Name: Anti-PP1C gamma Antibody
Brand: Antibodies.com
SKU: A14509
Price: 470.0 USD
Availability: InStock

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$470

Rabbit polyclonal antibody to PP1C gamma for WB and ICC/IF.

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Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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Knockout Validated Antibody

Specifications

Name

Anti-PP1C gamma Antibody

Description

Rabbit polyclonal antibody to PP1C gamma.

Applications

WB, ICC/IF

Dilutions

WB: 1:200-1:2,000, ICC/IF: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-323 of human PPP1CC (NP_002701.1).

Sequence

MADLDKLNIDSIIQRLLEVRGSKPGKNVQLQENEIRGLCLKSREIFLSQPILLELEAPLKICGDIHGQYYDLLRLFEYGGFPPESNYLFLGDYVDRGKQSLETICLLLAYKIKYPENFFLLRGNHECASINRIYGFYDECKRRYNIKLWKTFTDCFNCLPIAAIVDEKIFCCHGGLSPDLQSMEQIRRIMRPTDVPDQGLLCDLLWSDPDKDVLGWGENDRGVSFTFGAEVVAKFLHKHDLDLICRAHQVVEDGYEFFAKRQLVTLFSAPNYCGEFDNAGAMMSVDETLMCSFQILKPAEKKKPNATRPVTPPRGMITKQAKK

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

38 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

PP 1G, PP-1G, PP1C, PP1G, PP1gamma, PP1G_HUMAN, PPP 1G, PPP1CC, PPP1G, Protein phosphatase 1, catalytic subunit, gamma isozyme, Protein phosphatase 1C catalytic subunit, Serine/threonine phosphatase 1 gamma, Serine/threonine protein phosphatase PP1 gamma catalytic subunit, Serine/threonine-protein phosphatase PP1-gamma catalytic subunit

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (5)

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Figure 1: Western Blot - Anti-PP1C gamma Antibody (A14509)

Western blot analysis of extracts of various cell lines, using Anti-PP1C gamma Antibody (A14509) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 90s.

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Figure 2: Western Blot - Anti-PP1C gamma Antibody (A14509)

Western blot analysis of extracts from normal (control) and PPP1CC knockout (KO) HeLa cells, using Anti-PP1C gamma Antibody (A14509) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 1s.

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Figure 3: Immunofluorescence - Anti-PP1C gamma Antibody (A14509)

Immunofluorescence analysis of H9C2 cells using Anti-PP1C gamma Antibody (A14509) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).

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Figure 4: Immunofluorescence - Anti-PP1C gamma Antibody (A14509)

Immunofluorescence analysis of L929 cells using Anti-PP1C gamma Antibody (A14509) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).