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Anti-PPCS Antibody [ARC2889] (A306432)

Western Blot - Anti-PPCS Antibody [ARC2889] (A306432) - Antibodies.com Immunohistochemistry - Anti-PPCS Antibody [ARC2889] (A306432) - Antibodies.com Immunohistochemistry - Anti-PPCS Antibody [ARC2889] (A306432) - Antibodies.com Immunohistochemistry - Anti-PPCS Antibody [ARC2889] (A306432) - Antibodies.com
This product is discontinued

Anti-PPCS Antibody [ARC2889] (A306432) has been discontinued and is no longer available.

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Name
Anti-PPCS Antibody [ARC2889]
Description
Rabbit monoclonal [ARC2889] antibody to PPCS.
Applications
WB, IHC
Dilutions
WB: 1:100-1:500, IHC: 1:50-1:200
Reactivity
Human, Mouse, Rat
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 212-311 of human PPCS (Q9HAB8).
Sequence
KLLSPLVKDWAPKAFIISFKLETDPAIVINRARKALEIYQHQVVVANILESRQSFVFIVTKDSETKLLLSEEEIEKGVEIEEKIVDNLQSRHTAFIGDRN
Host
Rabbit
Clonality
Monoclonal
Clone ID
ARC2889
Isotype
IgG
Conjugate

Unconjugated

Purification
Affinity purification.
Molecular Weight
34 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.02% Sodium Azide.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Synonyms
COAB, FLJ11838, MGC117357, MGC138220, OTTHUMP00000008433, Phosphopantothenate--cysteine ligase, Phosphopantothenoylcysteine synthetase, PPC synthetase, PPCS_HUMAN, RP11-163G10.1
Isotype Controls
Suitable Secondaries
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
Western Blot - Anti-PPCS Antibody [ARC2889] (A306432) - Antibodies.comEnlarge Image
Figure 1: Western Blot - Anti-PPCS Antibody [ARC2889] (A306432)
Western blot analysis of extracts of various cell lines, using Anti-PPCS Antibody [ARC2889] (A306432) at 1:500 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 30s.
Immunohistochemistry - Anti-PPCS Antibody [ARC2889] (A306432) - Antibodies.comEnlarge Image
Figure 2: Immunohistochemistry - Anti-PPCS Antibody [ARC2889] (A306432)
Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue using Anti-PPCS Antibody [ARC2889] (A306432) at a dilution of 1:20 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunohistochemistry - Anti-PPCS Antibody [ARC2889] (A306432) - Antibodies.comEnlarge Image
Figure 3: Immunohistochemistry - Anti-PPCS Antibody [ARC2889] (A306432)
Immunohistochemistry analysis of paraffin-embedded mouse brain using Anti-PPCS Antibody [ARC2889] (A306432) at a dilution of 1:20 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunohistochemistry - Anti-PPCS Antibody [ARC2889] (A306432) - Antibodies.comEnlarge Image
Figure 4: Immunohistochemistry - Anti-PPCS Antibody [ARC2889] (A306432)
Immunohistochemistry analysis of paraffin-embedded rat brain using Anti-PPCS Antibody [ARC2889] (A306432) at a dilution of 1:20 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Publishing research using Anti-PPCS Antibody [ARC2889] (A306432)? Please let us know so that we can list the citation on this page.
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