Anti-PPM1A Antibody (A15333)

Name: Anti-PPM1A Antibody
Brand: Antibodies.com
SKU: A15333
Price: 335.0 USD
Availability: InStock

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$335

Rabbit polyclonal antibody to PPM1A for WB and ICC/IF.

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Delivery

Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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Telephone

+1 (314) 370-6046

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Specifications

Name

Anti-PPM1A Antibody

Description

Rabbit polyclonal antibody to PPM1A.

Applications

WB, ICC/IF

Dilutions

WB: 1:500-1:2,000, ICC/IF: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-382 of human PPM1A (NP_066283.1).

Sequence

MGAFLDKPKMEKHNAQGQGNGLRYGLSSMQGWRVEMEDAHTAVIGLPSGLESWSFFAVYDGHAGSQVAKYCCEHLLDHITNNQDFKGSAGAPSVENVKNGIRTGFLEIDEHMRVMSEKKHGADRSGSTAVGVLISPQHTYFINCGDSRGLLCRNRKVHFFTQDHKPSNPLEKERIQNAGGSVMIQRVNGSLAVSRALGDFDYKCVHGKGPTEQLVSPEPEVHDIERSEEDDQFIILACDGIWDVMGNEELCDFVRSRLEVTDDLEKVCNEVVDTCLYKGSRDNMSVILICFPNAPKVSPEAVKKEAELDKYLECRVEEIIKKQGEGVPDLVHVMRTLASENIPSLPPGGELASKRNVIEAVYNRLNPYKNDDTDSTSTDDMW

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

42 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

EC 3.1.3.16, FLJ42306, IA, MGC9201, Mpp alpha, PP2C alpha, PP2C-alpha, PP2CA, PP2Calpha, PPM 1A, PPM1A_HUMAN, PPPM1A, Protein phosphatase 1A, Protein phosphatase 1A (formerly 2C) magnesium dependent alpha isoform, Protein phosphatase 1A magnesium dependent alpha, Protein phosphatase 2C alpha, Protein phosphatase 2C alpha isoform, Protein phosphatase 2C isoform alpha, Protein phosphatase IA, Protein phosphatase, Mg2+/Mn2+ dependent, 1A

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (4)

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Figure 1: Western Blot - Anti-PPM1A Antibody (A15333)

Western blot analysis of extracts of various cell lines, using Anti-PPM1A Antibody (A15333) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 1s.

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Figure 2: Immunofluorescence - Anti-PPM1A Antibody (A15333)

Immunofluorescence analysis of C6 cells using Anti-PPM1A Antibody (A15333) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).

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Figure 3: Immunofluorescence - Anti-PPM1A Antibody (A15333)

Immunofluorescence analysis of HeLa cells using Anti-PPM1A Antibody (A15333) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).

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Figure 4: Immunofluorescence - Anti-PPM1A Antibody (A15333)

Immunofluorescence analysis of NIH-3T3 cells using Anti-PPM1A Antibody (A15333) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).

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