Anti-PPP1R15A Antibody (A83758)

Name: Anti-PPP1R15A Antibody
Brand: Antibodies.com
SKU: A83758
Price: 480.0 USD
Availability: InStock

This product is identical to Novus (NB100-778). Discover more for less.

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$480

Goat polyclonal antibody to PPP1R15A for ELISA, WB, IHC, IF and Flow Cytometry.

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Delivery

Freight Charges

$40 to United States

Lead Time

3-5 Business Days

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Telephone

+1 (314) 370-6046

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Specifications

Name

Anti-PPP1R15A Antibody

Description

Goat polyclonal antibody to PPP1R15A.

Applications

ELISA, WB, IHC, IF, Flow Cytometry

Reactivity

Human

Immunogen

Synthetic peptide corresponding to Human PPP1R15A (C terminal).

Sequence

C-AAALDLSGRRG

Host

Goat

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Concentration

100 µg at 0.5 mg/ml.

Product Form

Liquid

Formulation

Supplied in Tris Buffered Saline, pH 7.30, with 0.02% Sodium Azide and 0.5% BSA.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

Apoptosis associated protein, GADD 34, Growth arrest and DNA damage inducible 34, Growth arrest and DNA damage-inducible protein GADD34, Myeloid differentiation primary response protein MyD116 homolog, Ppp1r15a, PR15A_HUMAN, Protein phosphatase 1 regulatory (inhibitor) subunit 15A, Protein phosphatase 1 regulatory subunit 15A

Isotype Controls

Goat IgG (A121671)

Suitable Secondaries

See all Anti-Goat IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (5)

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Figure 1: Western Blot - Anti-PPP1R15A Antibody (A83758)

Anti-PPP1R15A Antibody (A83758) (0.3µg/ml) staining of HepG2 (A) and negative control KLY (B) lysate (35µg protein in RIPA buffer) Detected by chemiluminescence.

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Figure 2: Immunohistochemistry - Anti-PPP1R15A Antibody (A83758)

Anti-PPP1R15A Antibody (A83758) (2µg/ml) staining of paraffin embedded Human Liver. Steamed antigen retrieval with citrate buffer pH 6, HRP-staining.

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Figure 3: Flow Cytometry - Anti-PPP1R15A Antibody (A83758)

Anti-PPP1R15A Antibody (A83758) Flow cytometric analysis of paraformaldehyde fixed HepG2 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10µg/ml) followed by Alexa Fluor 488 secondary antibody (0.4µg/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.

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Figure 4: Immunofluorescence - Anti-PPP1R15A Antibody (A83758)

Anti-PPP1R15A Antibody (A83758) Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10µg/ml) followed by Alexa Fluor 488 secondary antibody (4µg/ml), showing strong nuclear and some cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10µg/ml) followed by Alexa Fluor 488 secondary antibody (4µg/ml).