Anti-PRAS40 Antibody (A11941)

Name: Anti-PRAS40 Antibody
Brand: Antibodies.com
SKU: A11941
Price: 335.0 USD
Availability: InStock

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$335

Rabbit polyclonal antibody to PRAS40 for WB, IHC and ICC/IF.

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Delivery

Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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Telephone

+1 (314) 370-6046

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Specifications

Name

Anti-PRAS40 Antibody

Description

Rabbit polyclonal antibody to PRAS40.

Applications

WB, IHC, ICC/IF

Dilutions

WB: 1:500-1:2,000, IHC: 1:50-1:100, ICC/IF: 1:50-1:100

Reactivity

Human, Mouse, Rat

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 150-256 of human AKT1S1/PRAS40 (NP_001092103.1).

Sequence

STDDGSLSEETPAGPPTCSVPPASALPTQQYAKSLPVSVPVWGFKEKRTEARSSDEENGPPSSPDLDRIAASMRALVLREAEDTQVFGDLPRPRLNTSDFQKLKRKY

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

50 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.01% Thiomersal.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

40 kDa proline rich AKT substrate, 40 kDa proline-rich AKT substrate, AKT1 S1, AKT1 substrate 1, AKT1 substrate 1 (proline rich), AKT1S 1, AKT1S1, AKTS1_HUMAN, Lobe, MGC2865, PRAS, PRAS 40, Proline rich akt substrate, Proline rich Akt substrate 40 kDa, Proline-rich AKT1 substrate 1

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (4)

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Figure 1: Western Blot - Anti-PRAS40 Antibody (A11941)

Western blot analysis of extracts of various cell lines, using Anti-PRAS40 Antibody (A11941) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Enhanced Kit (RM00021). Exposure time: 90s.

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Figure 2: Immunohistochemistry - Anti-PRAS40 Antibody (A11941)

Immunohistochemistry analysis of paraffin-embedded human mammary cancer using Anti-PRAS40 Antibody (A11941) at a dilution of 1:200 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

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Figure 3: Immunohistochemistry - Anti-PRAS40 Antibody (A11941)

Immunohistochemistry analysis of paraffin-embedded human stomach using Anti-PRAS40 Antibody (A11941) at a dilution of 1:200 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

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Figure 4: Immunofluorescence - Anti-PRAS40 Antibody (A11941)

Immunofluorescence analysis of NIH-3T3 cells using Anti-PRAS40 Antibody (A11941) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).

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