Anti-Prdm9 Antibody [ARC51589] (A305643)

Name: Anti-Prdm9 Antibody [ARC51589]
Brand: Antibodies.com
SKU: A305643

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This product is discontinued

Anti-Prdm9 Antibody [ARC51589] (A305643) has been discontinued and is no longer available.

View all Anti-Prdm9 Antibodies.

Specifications

Name

Anti-Prdm9 Antibody [ARC51589]

Description

Rabbit monoclonal [ARC51589] antibody to Prdm9.

Applications

WB, IHC

Dilutions

WB: 1:500-1:1,000, IHC: 1:500-1:1,000

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 87-370 of mouse Prdm9 (NP_659058.3).

Sequence

DSEDSDEEWTPKQQVSPPWVPFRVKHSKQQKESSRMPFSGESNVKEGSGIENLLNTSGSEHVQKPVSSLEEGNTSGQHSGKKLKLRKKNVEVKMYRLRERKGLAYEEVSEPQDDDYLYCEKCQNFFIDSCPNHGPPLFVKDSMVDRGHPNHSVLSLPPGLRISPSGIPEAGLGVWNEASDLPVGLHFGPYEGQITEDEEAANSGYSWLITKGRNCYEYVDGQDESQANWMRYVNCARDDEEQNLVAFQYHRKIFYRTCRVIRPGCELLVWYGDEYGQELGIKWG

Host

Rabbit

Clonality

Monoclonal

Clone ID

ARC51589

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

110 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.05% Proclin 300.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

EC 2.1.1.43, Histone lysine N methyltransferase PRDM9, Histone-lysine N-methyltransferase PRDM9, MEISETZ, PFM6, PR domain containing 9, PR domain containing protein 9, PR domain zinc finger protein 9, PR domain-containing protein 9, PRDM 9, PRDM9_HUMAN

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (4)

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Figure 1: Western Blot - Anti-Prdm9 Antibody [ARC51589] (A305643)

Western blot analysis of extracts of various cell lines, using Anti-Prdm9 Antibody [ARC51589] (A305643) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 10s.

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Figure 2: Western Blot - Anti-Prdm9 Antibody [ARC51589] (A305643)

Western blot analysis of extracts of Rat kidney, using Anti-Prdm9 Antibody [ARC51589] (A305643) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 30s.

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Figure 3: Immunohistochemistry - Anti-Prdm9 Antibody [ARC51589] (A305643)

Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue using Anti-Prdm9 Antibody [ARC51589] (A305643) at a dilution of 1:800 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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Figure 4: Immunohistochemistry - Anti-Prdm9 Antibody [ARC51589] (A305643)

Immunohistochemistry analysis of paraffin-embedded human colon tissue using Anti-Prdm9 Antibody [ARC51589] (A305643) at a dilution of 1:800 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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