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Anti-AMPK alpha 1 (phospho Thr183) + AMPK alpha 2 (phospho Thr172) Antibody (A90745)

Western Blot - Anti-AMPK alpha 1 (phospho Thr183) + AMPK alpha 2 (phospho Thr172) Antibody (A90745) - Antibodies.com
This product is discontinued

Anti-AMPK alpha 1 (phospho Thr183) + AMPK alpha 2 (phospho Thr172) Antibody (A90745) has been discontinued and is no longer available.

View all available products.

  • Specifications

Name
Anti-AMPK alpha 1 (phospho Thr183) + AMPK alpha 2 (phospho Thr172) Antibody
Description
Rabbit polyclonal antibody to AMPK alpha 1 (phospho Thr183) + AMPK alpha 2 (phospho Thr172).
Applications
WB
Dilutions
WB: 1:500-1:2,000
Reactivity
Human, Rat
Immunogen
A synthetic phosphorylated peptide around T183 of human PRKAA1PRKAA1 (NP_006242.5).
Sequence
LRTSC
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugate

Unconjugated

Purification
Affinity purification.
Molecular Weight
62 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.01% Thiomersal.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Isotype Controls
Suitable Secondaries
See all Anti-Rabbit IgG Secondaries →
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.

  • Scientific Validation DataValidation Data (1)

Western Blot - Anti-AMPK alpha 1 (phospho Thr183) + AMPK alpha 2 (phospho Thr172) Antibody (A90745) - Antibodies.comEnlarge Image

Western Blot - Anti-AMPK alpha 1 (phospho Thr183) + AMPK alpha 2 (phospho Thr172) Antibody (A90745)

Western blot analysis of extracts of various cell lines, using Anti-AMPK alpha 1 (phospho Thr183) + AMPK alpha 2 (phospho Thr172) Antibody (A90745) at 1:1,000 dilution. 293T and C6 cells were treated by ATP(5 mM) at 30°C for 1 hour. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Enhanced Kit (RM00021). Exposure time: 90s.