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Anti-PRKAR2B Antibody [ARC2272] (A305991)

Western Blot - Anti-PRKAR2B Antibody [ARC2272] (A305991) - Antibodies.com Immunohistochemistry - Anti-PRKAR2B Antibody [ARC2272] (A305991) - Antibodies.com Immunohistochemistry - Anti-PRKAR2B Antibody [ARC2272] (A305991) - Antibodies.com
$470
Product Datasheet

Rabbit monoclonal [ARC2272] antibody to PRKAR2B for WB and IHC.

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$40 to United States
Lead Time
5-8 Business Days

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Name
Anti-PRKAR2B Antibody [ARC2272]
Description
Rabbit monoclonal [ARC2272] antibody to PRKAR2B.
Applications
WB, IHC
Dilutions
WB: 1:500-1:2,000, IHC: 1:50-1:200
Reactivity
Human, Mouse, Rat
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 319-418 of human PRKAR2B (P31323).
Sequence
GEVKITMKRKGKSEVEENGAVEIARCSRGQYFGELALVTNKPRAASAHAIGTVKCLAMDVQAFERLLGPCMEIMKRNIATYEEQLVALFGTNMDIVEPTA
Host
Rabbit
Clonality
Monoclonal
Clone ID
ARC2272
Isotype
IgG
Conjugate

Unconjugated

Purification
Affinity purification.
Molecular Weight
46 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.02% Sodium Azide.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Synonyms
AI451071, AW061005, cAMP dependent protein kinase type II beta regulatory chain, cAMP dependent protein kinase type II beta regulatory subunit, cAMP-dependent protein kinase type II-beta regulatory subunit, H RG363E19.2, KAP3_HUMAN, MGC116401, PKA 2 beta (regulatory subunit), Pkarb2, PRKAR 2, PRKAR2, Protein kinase cAMP dependent regulatory type II beta, RATDNA, RII beta, RII(beta), RIIbeta, WUGSC:H RG363E19.2
Isotype Controls
Suitable Secondaries
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
Western Blot - Anti-PRKAR2B Antibody [ARC2272] (A305991) - Antibodies.comEnlarge Image
Figure 1: Western Blot - Anti-PRKAR2B Antibody [ARC2272] (A305991)
Western blot analysis of extracts of various cell lines, using Anti-PRKAR2B Antibody [ARC2272] (A305991) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 180s.
Immunohistochemistry - Anti-PRKAR2B Antibody [ARC2272] (A305991) - Antibodies.comEnlarge Image
Figure 2: Immunohistochemistry - Anti-PRKAR2B Antibody [ARC2272] (A305991)
Immunohistochemistry analysis of paraffin-embedded mouse brain using Anti-PRKAR2B Antibody [ARC2272] (A305991) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry - Anti-PRKAR2B Antibody [ARC2272] (A305991) - Antibodies.comEnlarge Image
Figure 3: Immunohistochemistry - Anti-PRKAR2B Antibody [ARC2272] (A305991)
Immunohistochemistry analysis of paraffin-embedded rat ovary using Anti-PRKAR2B Antibody [ARC2272] (A305991) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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