43 results for "Primary Antibodies" and "S. pombe"

43 Products
Immunohistochemistry - Anti-PCNA Antibody [PC10] (A249578) - Antibodies.com
(3)
Immunohistochemistry - Anti-PCNA Antibody [PC10] - BSA and Azide free (A252758) - Antibodies.com
(3)
Immunohistochemistry - Anti-PCNA Antibody [SPM350] (A249578) - Antibodies.com
Immunohistochemistry - Anti-PCNA Antibody [SPM350] - BSA and Azide free (A252758) - Antibodies.com
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Detection of Nup98 protein by Western blotting using this antibody, 2H10. The sample is HeLa nuclear membrane fraction. The IgG was diluted 2,000 fold before use.
(8)
Western blot analysis of Rhp51 in the whole cell extracts. M: Molecular size markers (kD). Lane 1: Wild-type strain. Lane2: Rhp51 deletion mutant strain.
(2)
Immunofluorescene staining of Nup98 in HeLa cells using 13C2 , 21A10 , or 2H10 monoclonal antibodies.
(7)
Immunofluorescene staining of Nup98 in HeLa cells using 13C2 , 21A10 , or 2H10 monoclonal antibodies.
(7)
Identification of Rad21 protein in crude extract of S. pombe with anti-Rad21 antibody
Immunofluorescene staining of Nup98 in HeLa cells using 13C2 , 21A10 , or 2H10 monoclonal antibodies.
(5)
Detection of endogenous level of Psm1 protein by western blotting.
Immunoblot with anti-Sds22 antiserum of yeast extracts from (1) wild type strain HM123, (2) sds::ura4+ deletion mutant carrying pHR140-2 (ref.2).
(3)
Western blot of Mcm2 protein in S. pombe crude extract. Anti-Mcm2 Antibody was used at 1:2,000 dilution. Goat Anti-Rabbit IgG Antibody (HRP) was used as a secondary at 1:10,000 dilution. S. pombe extract (8µg).
Western blot of Mcm6 / Mis5 protein. Anti-Mcm6 Antibody was used at 1:500 dilution. Goat Anti-Rabbit IgG Antibody (HRP) was used at 1:10,000 dilution. S. pombe extract (8µg). The antibody detects a protein band of 110 kDa, slightly larger than the one predicted from the protein sequence (95.5kDa).
Western blot of Orc2 protein in S. pombe crude extract. Anti-Orc2 Antibody was used at 1:2,000 dilution. Goat Anti-Rabbit IgG Antibody (HRP) was used as a secondary at 1:10,000 dilution. S. pombe extract (8µg).
Western blot of Ssb2 protein. Crude extract of S.pombe strain MP111 (8µg) was separated on 12.5% SDS-PAGE and blotted to PVDF membrane. Membrane was blocked with 5% skim milk. Anti-Ssb2 Antibody was used at 1:1,000 dilution and Goat Anti-Rabbit IgG (HRP) was used at 1:10,000 dilution. The antibody detects a 30kDa band, corresponding to the size predicted from the protein sequence.
Western blot of Orc4 protein in S. pombe crude extract. Sample: S. pombe crude extract (8µg). Anti-Orc4 Antibody was used at 1:2,000 dilution. Goat Anti-Rabbit IgG Antibody (HRP) was used as a secondary at 1:10,000 dilution.
Identification of Cnd2 protein in the crude extract of S. pombe by western blotting with this antiserum
(2)
Immunoblot of wild-type and ?dis2 S.pombe cells using anti-dis2 antibody, ?D2F (ref.3). wt: wild type ?dis2: dis2 deletion mutant
(2)
Cellular location of Ppa1 and Ppa2
Western blot analysis of Rad22 in the whole cell extracts with affinity-purified antibody. M: Molecular weight markers (kD). Lane 1:Protein size markers (kDa). Lane 2: Extract of wild-type S. pombe. ** Indicates Rad22 protein band.
(2)
Immunoblotting of extracts of S. pombe cells transformed with the vector or plasmids carrying truncated genes (172, A, B, C, E) with anti-Dis3 antibodies. Polypeptides of expected molecular masses were detected (ref.1).
(2)
Identification of Pad1 protein in crude extracts by anti-Pad1 antiserum
Western blot of endogenous Psf1 protein. Anti-Psf1 Antibody was used at 1:500 dilution. Goat Anti-Rabbit IgG Antibody (HRP) was used as a secondary at 1:10,000 dilution. S. pombe extract (8µg). The antibody detects the 23 kDa band, corresponding to the predicted molecular mass of Psf1 protein.
Western blot analysis of Mrc1 in the whole cell extracts. Lane 1: mrc- strain Lane 2: Wild type strain
Western blot of Sna41 protein in S. pombe crude extract. Anti-Sna41 Antibody was used at 1:2,000 dilution. Goat Anti-Rabbit IgG Antibody (HRP) was used as a secondary at 1:10,000 dilution. Signal enhancer, Can Get Signal (TOYOBO), was used in primary and secondary antibody reactions. S. pombe extract (18µg).
Identification of histone H2B in the crude extract of fission yeast S. pombe with this anti body.
Western blot of Mcm5 protein in S. pombe crude extract. Anti-Mcm5 Antibody was used at 1:2,000 dilution. Goat Anti-Rabbit IgG Antibody (HRP) was used as a secondary at 1:10,000 dilution. S. pombe extract (8µg).
Western blot of Psf3 protein. Anti-Psf1 Antibody was used at 1:500 dilution. Goat Anti-Rabbit IgG Antibody (HRP) was used as a secondary at 1:10,000 dilution. S. pombe extract (8µg). Signal enhancer, Can Get Signal (TOYOBO), was used in primary and secondary antibody reactions. The antibody detects a protein band with molecular mass of 18 kDa, corresponding to the mass predicted from the amino acid sequence.
Western blot of Sld3 protein. Anti-Sld5 Antibody was used at 1:500 dilution. Goat Anti-Rabbit IgG Antibody (HRP) was used as a secondary at 1:10,000 dilution. S. pombe extract (8µg). Signal enhancer, Can Get Signal (TOYOBO), was used in primary and secondary antibody reactions. The antibody detects a 28 kDa band, slightly larger than the one predicted from the protein sequence.
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Detection of Swi6 protein by Western blotting using this antibody.
Western blot analysis of ßtubulin in the whole cell extracts of S. pombe (10?g). Ant-? tublin antibody: 1,000 fold dilution
Identification of the Cut5/Rad4 protein in the crude extract of S. pombe with this antibody
Western blot of Mcm7 protein in S. pombe crude extract. Anti-Mcm7 Antibody was used at 1:2,000 dilution. Goat Anti-Rabbit IgG Antibody (HRP) was used as a secondary at 1:10,000 dilution. S. pombe extract (8µg).
dentification of Sds23 protein.
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Identification of Dis2 phosphorylated at T316 by western blotting with the antibody.
Detection of Gcn5 protein in crude lysate of S. cerevisiae strain BY4741 by western blotting with anti-Gcn5 antibody. Anti-Gcn5 antibody was used at 1/500 dilution and 2nd antibody, goat anti-rabbit IgG antibody conjugated with HRP, was used at 1/5,000 dilution. Signal enhancer, “CanGet Sigbnal” (Toyobo, Osaka), was used. Numbers on the left are positions of protein bands in kDa. Molecular mass of Gcn5 is 51 kDa.
Western blot analysis with Anti-Cdc22 Antibody. Cdc22 protein (92 kDa) was detected by western blotting in S. pombe crude extract. 7.5 % SDS-PAGE was used. Anti-Cdc22 Antibody was used at 1:1,000 dilution and Anti-Rabbit IgG Antibody (HRP) was used as a secondary antibody at 1:10,000 dilution.
Western blot of Mcm3 protein in S. pombe crude extract. Anti-Mcm3 Antibody was used at 1:2,000 dilution. Goat Anti-Rabbit IgG Antibody (HRP) was used as a secondary at 1:10,000 dilution. S. pombe extract (8µg).

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