Proteins that indirectly associate with methylated DNA play roles in interpreting and translating the epigenetic information encoded by DNA methylation patterns. These proteins often mediate interactions between DNA methyltransferases, DNA demethylases, or methyl-binding proteins, and other chromatin modifiers or transcriptional machinery. For example, Sin3A (Switch-Independent 3A) is a transcriptional corepressor that indirectly associates with methylated DNA regions through interactions with methyl-binding proteins like MeCP2. Sin3A interacts with MeCP2, which, in turn, binds to methylated DNA. This complex can then recruit histone deacetylases (HDACs), leading to the deacetylation of histones and chromatin condensation. Sin3A therefore contributes to gene repression by promoting a repressive chromatin environment and is involved in silencing genes that have methylated CpG islands in their promoters. CoREST (Co-Repressor for Element-1 Silencing Transcription Factor) is another corepressor that interacts with MeCP2 and associates indirectly with methylated DNA. CoREST forms a complex with MeCP2, and this complex can also recruit histone-modifying enzymes, such as HDACs and LSD1 (Lysine-specific demethylase 1). CoREST contributes to gene silencing by removing acetyl groups from histones and demethylating specific lysine residues on histones, leading to transcriptional repression. HP1 (Heterochromatin Protein 1) is a chromatin-associated protein that also indirectly interacts with methylated DNA through its association with MBD proteins. MBD proteins, such as MBD1 and MBD2, recruit HP1 to methylated chromatin. HP1 is known to bind to histone H3 methylated at lysine 9 (H3K9me3), a histone modification associated with heterochromatin. HP1 contributes to the formation of heterochromatin, a transcriptionally repressive chromatin state, by maintaining gene silencing. DNMT3L (DNA Methyltransferase 3-Like) is an accessory protein that indirectly associates with methylated DNA by interacting with de novo DNA methyltransferases, such as DNMT3A and DNMT3B. DNMT3L interacts with DNMT3A and DNMT3B and enhances their DNA methylation activity. Whilst it does not directly bind to methylated DNA, it influences their targeting and function. DNMT3L is therefore involved in establishing de novo DNA methylation patterns during early development and gametogenesis, thereby influencing gene regulation in the germline. TET Proteins (Ten-Eleven Translocation Enzymes), including TET1, TET2, and TET3, indirectly associate with methylated DNA by catalysing the oxidation of 5-methylcytosine (5mC) to 5-hydroxymethylcytosine (5hmC) and other oxidized derivatives. TET enzymes do not directly bind to methylated DNA but actively modify 5mC bases within the DNA molecule, leading to the conversion of methylated cytosines into hydroxy-methylated forms. TET-mediated oxidation of 5mC and subsequent DNA repair processes contribute to DNA demethylation, which can result in changes in gene expression patterns. TET enzymes are therefore critical for active DNA demethylation and gene regulation. Thus, some proteins indirectly associate with methylated DNA through interactions with methyl-binding proteins, chromatin modifiers, or enzymes involved in DNA methylation and demethylation. By participating in these complexes and pathways, they contribute to the dynamic regulation of gene expression, chromatin structure, and epigenetic modifications, shaping the cellular and developmental processes in which DNA methylation is involved. We offer a comprehensive product range of research tools for studying methylated DNA associated proteins, including CTCF antibodies, Tet2 antibodies, PRDM9 antibodies, TET3 antibodies, and TET1 antibodies. Explore our full methylated DNA associated proteins product range below and discover more, for less.