Anti-Progesterone Receptor Antibody [ARC5121-01] (A309446)

Western blot analysis of extracts of T-47D cells, using Anti-Progesterone Receptor Antibody [ARC5121-01] (A309446) at 1:10,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 4s.

Western blot analysis of extracts of various cell lines, using Anti-Progesterone Receptor Antibody [ARC5121-01] (A309446) at 1:10,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Enhanced Kit (RM00021). Exposure time: 180s.