Anti-Prohibitin Antibody [ARC0007] (A305437)

Name: Anti-Prohibitin Antibody [ARC0007]
Brand: Antibodies.com
SKU: A305437
Price: 470.0 USD
Availability: InStock

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$470

Rabbit monoclonal [ARC0007] antibody to Prohibitin for WB and IHC.

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Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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Tags:

Mitochondrial Marker

Specifications

Name

Anti-Prohibitin Antibody [ARC0007]

Description

Rabbit monoclonal [ARC0007] antibody to Prohibitin.

Applications

WB, IHC

Dilutions

WB: 1:500-1:2,000, IHC: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 100-200 of human Prohibitin (P35232).

Sequence

ASQLPRIFTSIGEDYDERVLPSITTEILKSVVARFDAGELITQRELVSRQVSDDLTERAATFGLILDDVSLTHLTFGKEFTEAVEAKQVAQQEAERARFVV

Host

Rabbit

Clonality

Monoclonal

Clone ID

ARC0007

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

30 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

Epididymis luminal protein 215, Epididymis secretory sperm binding protein Li 54e, HEL 215, HEL S 54e, PHB, PHB1, PHB_HUMAN

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (4)

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Figure 1: Western Blot - Anti-Prohibitin Antibody [ARC0007] (A305437)

Western blot analysis of extracts of various cell lines, using Anti-Prohibitin Antibody [ARC0007] (A305437) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 5s.

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Figure 2: Immunohistochemistry - Anti-Prohibitin Antibody [ARC0007] (A305437)

Immunohistochemistry analysis of paraffin-embedded rat heart using Anti-Prohibitin Antibody [ARC0007] (A305437) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

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Figure 3: Immunohistochemistry - Anti-Prohibitin Antibody [ARC0007] (A305437)

Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue using Anti-Prohibitin Antibody [ARC0007] (A305437) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

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Figure 4: Immunohistochemistry - Anti-Prohibitin Antibody [ARC0007] (A305437)

Immunohistochemistry analysis of paraffin-embedded mouse kidney using Anti-Prohibitin Antibody [ARC0007] (A305437) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

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