Anti-Proteasome alpha3 (phospho Ser250) Antibody (A94781)

Name: Anti-Proteasome alpha3 (phospho Ser250) Antibody
Brand: Antibodies.com
SKU: A94781
Price: 185.0 USD
Availability: InStock

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$185

Rabbit polyclonal antibody to Proteasome alpha3 (phospho Ser250) for WB and ELISA.

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Delivery

Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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Telephone

+1 (314) 370-6046

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Specifications

Name

Anti-Proteasome alpha3 (phospho Ser250) Antibody

Description

Rabbit polyclonal antibody to Proteasome alpha3 (phospho Ser250).

Specificity

This antibody detects endogenous levels of Proteasome alpha3 only when phosphorylated at Ser250.

Applications

WB, ELISA

Dilutions

IHC: 1:50-1:100, ELISA: 1:10000

Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide derived from human Proteasome alpha3 around the phosphorylation site of Ser250 (amino acids 206-255).

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Purified from rabbit serum by antigen affinity chromatography using the immunizing phospho peptide.

Molecular Weight

28kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline (without Mg2+ and Ca2+), pH 7.4, with 150mM NaCl, 0.02% Sodium Azide, and 50% Glycerol.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

HC8, Macropain subunit C8, MGC12306, MGC32631, Multicatalytic endopeptidase complex subunit C8, Proteasome (prosome macropain) subunit alpha type 3, Proteasome alpha 3 subunit, Proteasome component C8, Proteasome subunit alpha type 3, Proteasome subunit alpha type-3, Proteasome subunit C8, PSA3_HUMAN, PSC8, psmA3

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (3)

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Figure 1: Western Blot - Anti-Proteasome alpha3 (phospho Ser250) Antibody (A94781)

Western blot analysis of lysates from NIH/3T3 cells treated with EGF 200ng/ml 30' using Anti-Proteasome alpha3 (phospho Ser250) Antibody. The right hand lane represents a negative control, where the antibody is blocked by the immunising peptide.

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Figure 2: Western Blot - Anti-Proteasome alpha3 (phospho Ser250) Antibody (A94781)

Western blot analysis of 3T3 cells using Anti-Proteasome alpha3 (phospho Ser250) Antibody.

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Figure 3: Immunohistochemistry - Anti-Proteasome alpha3 (phospho Ser250) Antibody (A94781)

Immunohistochemical analysis of paraffin-embedded human brain using Anti-Proteasome alpha3 (phospho Ser250) Antibody 1:100 (4°C overnight). The right hand panel represents a negative control, where the antibody was pre-incubated with the immunising peptide.

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