Anti-PRPF4 Antibody [ARC3034] (A309448)

Name: Anti-PRPF4 Antibody [ARC3034]
Brand: Antibodies.com
SKU: A309448

Enlarge Image

This product is discontinued

Anti-PRPF4 Antibody [ARC3034] (A309448) has been discontinued and is no longer available.

View all Anti-PRPF4 Antibodies.

Specifications

Name

Anti-PRPF4 Antibody [ARC3034]

Description

Rabbit monoclonal [ARC3034] antibody to PRPF4.

Applications

WB, IHC

Dilutions

WB: 1:500-1:1,000, IHC: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant protein of Human PRPF4.

Host

Rabbit

Clonality

Monoclonal

Clone ID

ARC3034

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

60 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

hPrp4, HPRP4P, PRP4, PRP4 homolog, PRP4 pre mRNA processing factor 4 homolog (yeast), PRP4/STK/WD splicing factor, Prp4p, PRP4_HUMAN, RP70, SNRNP60, U4/U6 small nuclear ribonucleoprotein Prp4, U4/U6 snRNP 60 kDa protein, WD splicing factor Prp4

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (6)

Enlarge Image

Figure 1: Western Blot - Anti-PRPF4 Antibody [ARC3034] (A309448)

Western blot analysis of extracts of various cell lines, using Anti-PRPF4 Antibody [ARC3034] (A309448) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 30s.

Enlarge Image

Figure 2: Western Blot - Anti-PRPF4 Antibody [ARC3034] (A309448)

Western blot analysis of extracts of Rat liver, using Anti-PRPF4 Antibody [ARC3034] (A309448) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 180s.

Enlarge Image

Figure 3: Immunohistochemistry - Anti-PRPF4 Antibody [ARC3034] (A309448)

Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue using Anti-PRPF4 Antibody [ARC3034] (A309448) at a dilution of 1:50(40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Enlarge Image

Figure 4: Immunohistochemistry - Anti-PRPF4 Antibody [ARC3034] (A309448)

Immunohistochemistry analysis of paraffin-embedded human esophagus using Anti-PRPF4 Antibody [ARC3034] (A309448) at a dilution of 1:50(40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.