Anti-PSAP Antibody [ARC58322] (A309449)

Name: Anti-PSAP Antibody [ARC58322]
Brand: Antibodies.com
SKU: A309449

Enlarge Image

This product is discontinued

Anti-PSAP Antibody [ARC58322] (A309449) has been discontinued and is no longer available.

View all Anti-PSAP Antibodies.

Specifications

Name

Anti-PSAP Antibody [ARC58322]

Description

Rabbit monoclonal [ARC58322] antibody to PSAP.

Applications

WB, IHC

Dilutions

WB: 1:500-1:1,000, IHC: 1:100-1:500

Reactivity

Human

Immunogen

Recombinant protein of human PSAP.

Host

Rabbit

Clonality

Monoclonal

Clone ID

ARC58322

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

72 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.05% Proclin 300.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

A1 activator, Cerebroside sulfate activator, Co-beta-glucosidase, Component C, CSAct, Dispersin, GLBA, Glucosylceramidase activator, Proactivator polypeptide, Proactivator polypeptide precursor, Prosaposin, Prosaposin (sphingolipid activator protein 1), prosaposin (variant Gaucher disease and variant metachromatic leukodystrophy), Protein A, Protein C, SAP-1, SAP-2, SAP1, Saposin A, Saposin B, Saposin B Val, Saposin C, Saposin D, Saposin-D, Saposins, SAP_HUMAN, Sgp1, Sphingolipid activator protein 1, Sphingolipid activator protein 2, Sulfated glycoprotein 1, Sulfatide/GM1 activator

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (3)

Enlarge Image

Figure 1: Western Blot - Anti-PSAP Antibody [ARC58322] (A309449)

Western blot analysis of HeLa, using Anti-PSAP Antibody [ARC58322] (A309449) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Enhanced Kit (RM00021). Exposure time: 60s.

Enlarge Image

Figure 2: Western Blot - Anti-PSAP Antibody [ARC58322] (A309449)

Western blot analysis of extracts from wild type(WT) and PSAP knockdown (KD) 293T(KD) cells, using Anti-PSAP Antibody [ARC58322] (A309449) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Enhanced Kit (RM00021). Exposure time: 60s.

Enlarge Image

Figure 3: Immunohistochemistry - Anti-PSAP Antibody [ARC58322] (A309449)

Immunohistochemistry analysis of paraffin-embedded human liver cancer using Anti-PSAP Antibody [ARC58322] (A309449) at a dilution of 1:400 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Publishing research using Anti-PSAP Antibody [ARC58322] (A309449)? Please let us know so that we can list the citation on this page.