Anti-PTEN Antibody [AMC0179] (A13234)

Name: Anti-PTEN Antibody [AMC0179]
Brand: Antibodies.com
SKU: A13234

Enlarge Image

This product is discontinued

Anti-PTEN Antibody [AMC0179] (A13234) has been discontinued and is no longer available.

View all Anti-PTEN Antibodies.

Specifications

Name

Anti-PTEN Antibody [AMC0179]

Description

Mouse monoclonal [AMC0179] antibody to PTEN.

Applications

Dilutions

WB: 1:500-1:1,000

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 204-403 of human PTEN (P60484).

Sequence

PMFSGGTCNPQFVVCQLKVKIYSSNSGPTRREDKFMYFEFPQPLPVCGDIKVEFFHKQNKMLKKDKMFHFWVNTFFIPGPEETSEKVENGSLCDQEIDSICSIERADNDKEYLVLTLTKNDLDKANKDKANRYFSPNFKVKLYFTKTVEEPSNPEASSSTSVTPDVSDNEPDHYRYSDTTDSDPENEPFDEDQHTQITKV

Host

Mouse

Clonality

Monoclonal

Clone ID

AMC0179

Isotype

IgG1

Light Chains

kappa

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

55 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.01% Thiomersal.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

10q23del, BZS, DEC, GLM2, MGC11227, MHAM, MMAC1, MMAC1 phosphatase and tensin homolog deleted on chromosome 10, Mutated in multiple advanced cancers 1, Phosphatase and tensin homolog, Phosphatase and tensin like protein, Phosphatidylinositol 3,4,5-trisphosphate 3-phosphatase and dual-specificity protein phosphatase PTEN, PTEN1, PTEN_HUMAN, TEP1

Isotype Controls

Suitable Secondaries

See all Anti-Mouse IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (2)

Enlarge Image

Figure 1: Western Blot - Anti-PTEN Antibody [AMC0179] (A13234)

Western blot analysis of extracts of various cell lines, using Anti-PTEN Antibody [AMC0179] (A13234) at 1:1,000 dilution. The secondary antibody was HRP Goat Anti-Mouse IgG (H+L) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 90s.

Enlarge Image

Figure 2: Western Blot - Anti-PTEN Antibody [AMC0179] (A13234)

Western blot analysis of extracts of 293T cells, using Anti-PTEN Antibody [AMC0179] (A13234) at 1:1,000 dilution. The secondary antibody was HRP Goat Anti-Mouse IgG (H+L) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 180s.

Publishing research using Anti-PTEN Antibody [AMC0179] (A13234)? Please let us know so that we can list the citation on this page.