Anti-RAB10 Antibody [ARC55653] (A309454)

Name: Anti-RAB10 Antibody [ARC55653]
Brand: Antibodies.com
SKU: A309454

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This product is discontinued

Anti-RAB10 Antibody [ARC55653] (A309454) has been discontinued and is no longer available.

View all Anti-RAB10 Antibodies.

Specifications

Name

Anti-RAB10 Antibody [ARC55653]

Description

Rabbit monoclonal [ARC55653] antibody to RAB10.

Applications

WB, ICC/IF

Dilutions

WB: 1:10,000-1:70,000, ICC/IF: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant protein of human RAB10.

Host

Rabbit

Clonality

Monoclonal

Clone ID

ARC55653

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

23 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.05% Proclin 300.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

GTP binding protein RAB10, RAB10 member RAS oncogene family, RAB10_HUMAN, Ras related GTP binding protein, Ras related GTP binding protein RAB10, Ras-related protein Rab-10

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (4)

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Figure 1: Western Blot - Anti-RAB10 Antibody [ARC55653] (A309454)

Western blot analysis of various lysates, using Anti-RAB10 Antibody [ARC55653] (A309454) at 1:60,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 180s.

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Figure 2: Western Blot - Anti-RAB10 Antibody [ARC55653] (A309454)

Western blot analysis of extracts from wild type(WT) and RAB10 knockdown (KD) U-87MG(KD) cells, using Anti-RAB10 Antibody [ARC55653] (A309454) at 1:60,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 180s.

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Figure 3: Immunofluorescence - Anti-RAB10 Antibody [ARC55653] (A309454)

Immunofluorescence analysis of MCF7 using Anti-RAB10 Antibody [ARC55653] (A309454) at a dilution of 1:200 (40x lens). DAPI was used to stain the cell nuclei (blue).

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Figure 4: Immunofluorescence - Anti-RAB10 Antibody [ARC55653] (A309454)

Immunofluorescence analysis of NIH/3T3 using Anti-RAB10 Antibody [ARC55653] (A309454) at a dilution of 1:200 (40x lens). DAPI was used to stain the cell nuclei (blue).

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