Anti-Rab5b Antibody (A15705)

Name: Anti-Rab5b Antibody
Brand: Antibodies.com
SKU: A15705
Price: 335.0 USD
Availability: InStock

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$335

Rabbit polyclonal antibody to Rab5b for WB and ICC/IF.

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Delivery

Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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Telephone

+1 (314) 370-6046

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Specifications

Name

Anti-Rab5b Antibody

Description

Rabbit polyclonal antibody to Rab5b.

Applications

WB, ICC/IF

Dilutions

WB: 1:500-1:2,000, ICC/IF: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 100-200 of human RAB5B (NP_002859.1).

Sequence

YDITNQETFARAKTWVKELQRQASPSIVIALAGNKADLANKRMVEYEEAQAYADDNSLLFMETSAKTAMNVNDLFLAIAKKLPKSEPQNLGGAAGRSRGVD

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

24 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.01% Thiomersal.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

Rab 5b, Rab5 b, RAB5B, member RAS oncogene family, Ras related protein Rab 5B, RAS-associated protein RAB5B

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (3)

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Figure 1: Western Blot - Anti-Rab5b Antibody (A15705)

Western blot analysis of extracts of various cell lines, using Anti-Rab5b Antibody (A15705) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Enhanced Kit (RM00021). Exposure time: 90s.

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Figure 2: Immunofluorescence - Anti-Rab5b Antibody (A15705)

Immunofluorescence analysis of L929 cells using Anti-Rab5b Antibody (A15705) at a dilution of 1:100. DAPI was used to stain the cell nuclei (blue).

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Figure 3: Immunofluorescence - Anti-Rab5b Antibody (A15705)

Immunofluorescence analysis of human colon carcinoma using Anti-Rab5b Antibody (A15705) at a dilution of 1:100. DAPI was used to stain the cell nuclei (blue).

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