Anti-RAB8A Antibody [ARC2968] (A308507)

Name: Anti-RAB8A Antibody [ARC2968]
Brand: Antibodies.com
SKU: A308507

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This product is discontinued

Anti-RAB8A Antibody [ARC2968] (A308507) has been discontinued and is no longer available.

View all Anti-RAB8A Antibodies.

Specifications

Name

Anti-RAB8A Antibody [ARC2968]

Description

Rabbit monoclonal [ARC2968] antibody to RAB8A.

Applications

WB, ICC/IF, IP

Dilutions

WB: 1:500-1:1,000, ICC/IF: 1:50-1:200, IP: 1:500-1:1,000

Reactivity

Human, Mouse, Rat

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 100-207 of human RAB8A (P61006).

Sequence

RNWIRNIEEHASADVEKMILGNKCDVNDKRQVSKERGEKLALDYGIKFMETSAKANINVENAFFTLARDIKAKMDKKLEGNSPQGSNQGVKITPDQQKRSSFFRCVLL

Host

Rabbit

Clonality

Monoclonal

Clone ID

ARC2968

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

24 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

AA409338, MEL, Mel transforming oncogene, Mel transforming oncogene (derived from cell line NK14), Mel transforming oncogene (derived from cell line NK14) RAB8 homolog, Mel transforming oncogene (RAB8 homolog), MGC124948, Oncogene c mel, Oncogene c-mel, RAB8, RAB8A member RAS oncogene family, RAB8A_HUMAN, Ras associated protein RAB8, Ras related protein Rab 8A, Ras-related protein Rab-8A

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (5)

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Figure 1: Western Blot - Anti-RAB8A Antibody [ARC2968] (A308507)

Western blot analysis of extracts of various cell lines, using Anti-RAB8A Antibody [ARC2968] (A308507) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 3s.

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Figure 2: Western Blot - Anti-RAB8A Antibody [ARC2968] (A308507)

Western blot analysis of extracts of Rat spinal cord, using Anti-RAB8A Antibody [ARC2968] (A308507) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 60s.

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Figure 3: Immunofluorescence - Anti-RAB8A Antibody [ARC2968] (A308507)

Immunofluorescence analysis of NIH/3T3 cells using Anti-RAB8A Antibody [ARC2968] (A308507) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).

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Figure 4: Immunofluorescence - Anti-RAB8A Antibody [ARC2968] (A308507)

Immunofluorescence analysis of PC-12 cells using Anti-RAB8A Antibody [ARC2968] (A308507) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).