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Recombinant Anti-Rabbit IgG F(ab) Antibody [RMG01] (Biotin) (A121276)

Western Blot - Anti-Rabbit IgG F(ab) Antibody (Biotin) [RMG01] (A121275) - Antibodies.com ELISA - Anti-Rabbit IgG F(ab) Antibody (Biotin) [RMG01] (A121276) - Antibodies.com ELISA - Anti-Rabbit IgG F(ab) Antibody (Biotin) [RMG01] (A121275) - Antibodies.com
$525

Recombinant goat monoclonal [RMG01] antibody to Rabbit IgG F(ab) (Biotin) for IP, ICC, IHC, Flow Cytometry, ELISA and WB (non-reduced).

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$40 to United States
Lead Time
4-6 Business Days

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Name
Anti-Rabbit IgG F(ab) Antibody [RMG01] (Biotin)
Description
Recombinant goat monoclonal [RMG01] antibody to Rabbit IgG F(ab) (Biotin).
Host Species
Goat
Target Species
Rabbit
Specificity
This antibody recognizes the Fab region of rabbit IgG.
Applications
IP, ICC, IHC, Flow Cytometry, ELISA, WB (non-reduced)
Recommended Dilutions
ELISA: 0.01-0.5 µg/ml, ICC: 0.5-2 µg/ml, IHC: 0.5-2 µg/ml, WB: 0.10-5 µg/ml
Immunogen
Rabbit IgG.
Antigen Species
Rabbit
Clonality
Monoclonal
Isotype
IgG
Conjugate

Biotin

Product Form
Liquid
Concentration
1 mg/ml
Purification
Protein G chromatography.
Formulation
Supplied in Phosphate Buffered Saline with 50% Glycerol, 1% BSA, and 0.09% Sodium Azide.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
Western Blot - Anti-Rabbit IgG F(ab) Antibody (Biotin) [RMG01] (A121275) - Antibodies.comEnlarge Image
Figure 1: Western Blot - Anti-Rabbit IgG F(ab) Antibody [RMG01] (Biotin) (A121276)
Western blot of non-reduced (-) and reduced (+) rabbit IgG (20ng/lane), using 0.2µg/ml of Anti-Rabbit IgG F(ab) Antibody [RMG01] (Biotin). This antibody reacts to non-reduced rabbit IgG (~150 kDa).
ELISA - Anti-Rabbit IgG F(ab) Antibody (Biotin) [RMG01] (A121276) - Antibodies.comEnlarge Image
Figure 2: Anti-Rabbit IgG F(ab) Antibody [RMG01] (Biotin) (A121276)
ELISA of IgGs from different species, shows Anti-Rabbit IgG F(ab) Antibody [RMG01] reacts to the Fab region of rabbit IgG. There is no cross reactivity with human IgG, rat IgG, or mouse IgG. The plate was coated with 50ng/well of different IgG. 500ng/ml, 200ng/ml, or 50ng/ml of Anti-Rabbit IgG F(ab) Antibody [RMG01] was used as the primary antibody. An alkaline phosphatase conjugated anti-goat IgG as the secondary antibody.
ELISA - Anti-Rabbit IgG F(ab) Antibody (Biotin) [RMG01] (A121275) - Antibodies.comEnlarge Image
Figure 3: Anti-Rabbit IgG F(ab) Antibody [RMG01] (Biotin) (A121276)
A titer ELISA of rabbit IgG. The plate was coated with different amounts of rabbit IgG. A serial dilution of Anti-Rabbit IgG F(ab) Antibody [RMG01] was used as the primary antibody. An alkaline phosphatase conjugated anti-goat IgG as the secondary antibody.
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