Rabbit Anti-Sheep IgG H&L Antibody (Biotin) (A282210)

$390

Rabbit anti-sheep IgG H&L (Biotin) secondary antibody for ELISA, WB, IHC-Fr and Flow Cytometry.

Freight Charges
Lead Time
4-6 Business Days
Telephone
+1 (314) 370-6046
Mon - Fri, 8am - 4pm AST
Email
orders@antibodies.com
Name
Rabbit Anti-Sheep IgG H&L Antibody (Biotin)
Description
Rabbit anti-sheep IgG H&L (Biotin) secondary antibody.
Host Species
Rabbit
Target Species
Sheep
Specificity
This antibody recognises intact ovine IgG, raised against the whole IgG molecule it may recognize epitpoes encompassing both heavy and light chains. The antibody has not been cross adsorbed and may react with immunoglobulins of other subtypes and from other species.Biotinylated Rabbit anti Sheep IgG polyclonal antibody is a useful and robust reagent for the detection of bound sheep antibodies with labeled streptavidin as a tertiary reagent.Biotinylated Rabbit anti Sheep IgG polyclonal antibody has been used successfully for evaluation of IgG responses in vaccinated and challenged sheep.
Applications
ELISA, WB, IHC-Fr, Flow Cytometry
Recommended Dilutions
ELISA: 1:20,000 - 1:400,000, Flow Cytometry: 1:200 - 1:1,000, IHC-Fr: 1:500 - 1:5,000, WB: 1:20,000 - 1:400,000
Immunogen
Whole sheep IgG.
Clonality
Polyclonal
Isotype
IgG
Conjugate

Biotin

Product Form
Lyophilized
Concentration
Reconstitution dependent.
Purification
Immunoaffinity chromatography.
Formulation
Supplied in Phosphate Buffered Saline with 0.05% Sodium Azide and 1.5% BSA.
Storage
Lyophilized: Store at 4°C. Reconstituted: Store at 4°C or -20°C, avoid freeze / thaw cycles. Storage in frost free freezers is not recommended.
General Notes
Biotinylated Rabbit anti Sheep IgG polyclonal antibody recognizes intact ovine IgG, raised against the whole IgG molecule it may recognize epitpoes encompassing both heavy and light chains. The antibody has not been cross adsorbed and may react with immunoglobulins of other subtypes and from other species.Biotinylated Rabbit anti Sheep IgG polyclonal antibody is a useful and robust reagent for the detection of bound sheep antibodies with labeled streptavidin as a tertiary reagent.Biotinylated Rabbit anti Sheep IgG polyclonal antibody has been used successfully for evaluation of IgG responses in vaccinated and challenged sheep (Ramos et al. 2018).
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
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