Anti-Rad21 Antibody [ARC2276] (A305731)

Name: Anti-Rad21 Antibody [ARC2276]
Brand: Antibodies.com
SKU: A305731
Price: 470.0 USD
Availability: InStock

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$470

Rabbit monoclonal [ARC2276] antibody to Rad21 for WB, IP and ChIP.

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Delivery

Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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Specifications

Name

Anti-Rad21 Antibody [ARC2276]

Description

Rabbit monoclonal [ARC2276] antibody to Rad21.

Applications

WB, IP, ChIP

Dilutions

WB: 1:500-1:2,000, ChIP: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 532-631 of human Rad21 (O60216).

Sequence

EKEDDEEEEDEDASGGDQDQEERRWNKRTQQMLHGLQRALAKTGAESISLLELCRNTNRKQAAAKFYSFLVLKKQQAIELTQEEPYSDIIATPGPRFHII

Host

Rabbit

Clonality

Monoclonal

Clone ID

ARC2276

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

130 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

CDLS4, Double-strand-break repair protein rad21 homolog, hHR21, HR21, HRAD21, KIAA0078, MCD1, Nuclear matrix protein 1, NXP-1, NXP1, Protein involved in DNA double-strand break repair, RAD21 homolog, RAD21 homolog (S. pombe), RAD21_HUMAN, Scc1, SCC1 homolog

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (2)

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Figure 1: Western Blot - Anti-Rad21 Antibody [ARC2276] (A305731)

Western blot analysis of extracts of various cell lines, using Anti-Rad21 Antibody [ARC2276] (A305731) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Enhanced Kit (RM00021). Exposure time: 180s.

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Figure 2: Chromatin Immunoprecipitation - Anti-Rad21 Antibody [ARC2276] (A305731)

Chromatin immunoprecipitation (ChIP) analysis of extracts of A-549 cells, using Anti-Rad21 Antibody [ARC2276] (A305731) and Rabbit IgG. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.

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