Anti-Rad6 Antibody (A15867)

Name: Anti-Rad6 Antibody
Brand: Antibodies.com
SKU: A15867
Price: 335.0 USD
Availability: InStock

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$335

Rabbit polyclonal antibody to Rad6 for WB and IHC.

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Delivery

Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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Telephone

+1 (314) 370-6046

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Specifications

Name

Anti-Rad6 Antibody

Description

Rabbit polyclonal antibody to Rad6.

Applications

WB, IHC

Dilutions

WB: 1:500-1:2,000, IHC: 1:50-1:100

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-152 of human UBE2A (NP_003327.2).

Sequence

MSTPARRRLMRDFKRLQEDPPAGVSGAPSENNIMVWNAVIFGPEGTPFEDGTFKLTIEFTEEYPNKPPTVRFVSKMFHPNVYADGSICLDILQNRWSPTYDVSSILTSIQSLLDEPNPNSPANSQAAQLYQENKREYEKRVSAIVEQSWRDC

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

14 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

BHR6A, hHR6A, HR6A, mHR6A, MRXS30, MRXSN, RAD6 homolog A, RAD6A, UBC2, UBCD6, UBE2A, Ube2a ubiquitin-conjugating enzyme E2A, UBE2A_HUMAN, Ubiquitin carrier protein, Ubiquitin carrier protein A, Ubiquitin conjugating enzyme E2 17 kDa, Ubiquitin conjugating enzyme E2 21.5 kDa, Ubiquitin conjugating enzyme E2A, Ubiquitin conjugating enzyme E2A (RAD6 homolog), Ubiquitin protein ligase A, Ubiquitin-conjugating enzyme E2 A, Ubiquitin-protein ligase A

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (4)

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Figure 1: Western Blot - Anti-Rad6 Antibody (A15867)

Western blot analysis of extracts of various cell lines, using Anti-Rad6 Antibody (A15867) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Enhanced Kit (RM00021). Exposure time: 90s.

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Figure 2: Immunohistochemistry - Anti-Rad6 Antibody (A15867)

Immunohistochemistry analysis of paraffin-embedded mouse stomach using Anti-Rad6 Antibody (A15867) (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

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Figure 3: Immunohistochemistry - Anti-Rad6 Antibody (A15867)

Immunohistochemistry analysis of paraffin-embedded rat brain using Anti-Rad6 Antibody (A15867) (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

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Figure 4: Immunohistochemistry - Anti-Rad6 Antibody (A15867)

Immunohistochemistry analysis of paraffin-embedded human esophagus using Anti-Rad6 Antibody (A15867) (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

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