Anti-Raf1 (phospho Ser621) Antibody [ARC1556] (A308602)

Name: Anti-Raf1 (phospho Ser621) Antibody [ARC1556]
Brand: Antibodies.com
SKU: A308602
Price: 540.0 USD
Availability: InStock

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$540

Rabbit monoclonal [ARC1556] antibody to Raf1 (phospho Ser621) for WB.

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Delivery

Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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Specifications

Name

Anti-Raf1 (phospho Ser621) Antibody [ARC1556]

Description

Rabbit monoclonal [ARC1556] antibody to Raf1 (phospho Ser621).

Applications

Dilutions

WB: 1:500-1:2,000

Reactivity

Human, Rat

Immunogen

A synthetic phosphorylated peptide around S621 of human Phospho-Raf1-S621 (P04049).

Sequence

SASEP

Host

Rabbit

Clonality

Monoclonal

Clone ID

ARC1556

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

74 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

c Raf, C-raf, C-Raf proto-oncogene, serine/threonine kinase, CMD1NN, cRaf, Craf 1 transforming gene, Craf1 transforming gene, EC 2.7.11.1, kinase Raf1, Murine sarcoma 3611 oncogene 1, NS5, Oncogene MIL, Oncogene RAF1, OTTHUMP00000160218, OTTHUMP00000207813, OTTHUMP00000209389, Protein kinase raf 1, Proto-oncogene c-RAF, RAF, Raf 1, Raf 1 proto oncogene serine/threonine kinase, Raf proto oncogene serine/threonine protein kinase, RAF proto-oncogene serine/threonine-protein kinase, RAF-1, RAF1_HUMAN, Similar to murine leukemia viral (V-raf-1) oncogene homolog 1, TRANSFORMING REPLICATION-DEFECTIVE MURINE RETROVIRUS 3611-MSV, v raf 1 murine leukemia viral oncogene homolog 1, v-raf murine sarcoma viral oncogene homolog 1, v-raf-1 murine leukemia viral oncogene-like protein 1, vraf1 murine leukemia viral oncogene homolog 1

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (2)

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Figure 1: Western Blot - Anti-Raf1 (phospho Ser621) Antibody [ARC1556] (A308602)

Western blot analysis of extracts of C6 cells, using Anti-Raf1 (phospho Ser621) Antibody [ARC1556] (A308602) at 1:1,000 dilution. C6 cells were treated by CIP(20uL/400ul) at 37°C for 1 hour. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% BSA. Detection was with a ECL Basic Kit. Exposure time: 3min.

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Figure 2: Western Blot - Anti-Raf1 (phospho Ser621) Antibody [ARC1556] (A308602)

Western blot analysis of extracts of HeLa cells, using Anti-Raf1 (phospho Ser621) Antibody [ARC1556] (A308602) at 1:1,000 dilution. HeLa cells were treated by CIP(20uL/400ul) at 37°C for 1 hour. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% BSA. Detection was with a ECL Enhanced Kit (RM00021). Exposure time: 3min.

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