Anti-RAGE Antibody [ARC60991] (A309934)

Name: Anti-RAGE Antibody [ARC60991]
Brand: Antibodies.com
SKU: A309934

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This product is discontinued

Anti-RAGE Antibody [ARC60991] (A309934) has been discontinued and is no longer available.

View all Anti-RAGE Antibodies.

Specifications

Name

Anti-RAGE Antibody [ARC60991]

Description

Rabbit monoclonal [ARC60991] antibody to RAGE.

Applications

WB, IHC, ICC/IF

Dilutions

WB: 1:1,000-1:5,000, IHC: 1:50-1:200, IF: 1:50-1:100

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant protein of human AGER.

Host

Rabbit

Clonality

Monoclonal

Clone ID

ARC60991

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

58 kDa / 52 kDa / 48 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.05% Proclin 300.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

Advanced glycosylation end product-specific receptor, Ager, MGC2235, RAGE_HUMAN, Receptor for advanced glycosylation end products

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (2)

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Figure 1: Western Blot - Anti-RAGE Antibody [ARC60991] (A309934)

Western blot analysis of Rat lung, using Anti-RAGE Antibody [ARC60991] (A309934) at 1:2,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 60s.

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Figure 2: Immunohistochemistry - Anti-RAGE Antibody [ARC60991] (A309934)

Immunohistochemistry analysis of paraffin-embedded human lung using Anti-RAGE Antibody [ARC60991] (A309934) at a dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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