Anti-RAS Antibody (A305081)

Name: Anti-RAS Antibody
Brand: Antibodies.com
SKU: A305081
Price: 435.0 USD
Availability: InStock

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$435

Rabbit polyclonal antibody to RAS for WB, IHC and ICC/IF.

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Delivery

Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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+1 (314) 370-6046

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Specifications

Name

Anti-RAS Antibody

Description

Rabbit polyclonal antibody to RAS.

Applications

WB, IHC, ICC/IF

Dilutions

WB: 1:1,000, IHC: 1:100, ICC/IF: 1:200

Reactivity

Human, Mouse, Rat, Bovine

Immunogen

Synthetic peptide corresponding to amino acids 31-43 of human RAS. (The immunogen peptide is from the effector binding loop (l2) of the H, K and N-Ras (AA31-43). This sequence is identical yeast, slime mould, fungi, Xenopus, rat, mouse and chicken over these residues.).

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Protein G purification.

Concentration

1 mg/ml

Molecular Weight

~21 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.4, with 50% Glycerol and 0.09% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

C-BAS/HAS, c-H-ras, C-HA-RAS1, CTLO, GTPase HRas, GTPase KRas, GTPase NRas, H-Ras-1, H-RASIDX, Ha-Ras, HAMSV, HRAS, HRAS1, K RAS2A, K RAS2B, K RAS4A, K RAS4B, K-RAS, KRAS, KRAS1, KRAS2, N-RAS, N-terminally processed, NRAS, NRAS1, p21ras, RASH1, RASH_HUMAN, RASK2, Transforming protein p21, v Ha ras Harvey rat sarcoma viral oncogene homolog, v Ki ras2 Kirsten rat sarcoma viral oncogene homolog, v ras neuroblastoma RAS viral oncogene homolog

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (5)

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Figure 1: Western Blot - Anti-RAS Antibody (A305081)

Western blot analysis of mouse brain Cortex cell lysates showing detection of ~21 kDa RAS protein using Anti-RAS Antibody (A305081) at 1:1,000 for 2 hours at room temperature. Lane 1: Molecular Weight Ladder (MW). Lane 2: mouse brain Cortex cell lysates. Load: 30 µg. Block: 5% Skim Milk in 1X TBST. The secondary antibody used was Goat Anti-Rabbit IgG: HRP at 1:2000 for 60 minutes at room temperature. Color Development: ECL solution for 5 minutes at room temperature. Predicted/Observed Size: ~21 kDa.

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Figure 2: Immunofluorescence - Anti-RAS Antibody (A305081)

Immunocytochemistry/Immunofluorescence analysis of human cervical cancer cell line (HeLa), fixed in 2% formaldehyde for 20 minutes at room temperature, using Anti-RAS Antibody (A305081), at 1:200 for 12 hours at 4°C. The secondary antibody used was FITC Goat Anti-Rabbit (green) at 1:200 for 2 hours at room temperature. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at room temperature. Localization: Cell membrane. Lipid-anchor. Focal adhesion. Magnification: 20x.(A) DAPI (blue) nuclear stain. (B) Anti-RAS Antibody. (C) Composite.

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Figure 3: Immunofluorescence - Anti-RAS Antibody (A305081)

Immunocytochemistry/Immunofluorescence analysis of human HaCaT cells, fixed in cold 100% methanol at -20°C for 10 minutes, using Anti-RAS Antibody (A305081), at 1:100 for 12 hours at 4°C. The secondary antibody used was FITC Goat Anti-Rabbit at 1:50 for 1-2 hours at room temperature in dark. Localization: Cell-cell junction.

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Figure 4: Immunohistochemistry - Anti-RAS Antibody (A305081)

Immunohistochemistry analysis of mouse backskin, fixed in Bouin's fixative solution. The Primary Antibody used was Anti-RAS Antibody (A305081) at 1:100 for 1 hour at room temperature. The secondary antibody used was FITC Goat Anti-Rabbit (green) at 1:50 for 1 hour at room temperature. Localization: Basal cell of the epidermis.