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Anti-RASGRP1 Antibody (A8633)

Western Blot - Anti-RASGRP1 Antibody (A8633) - Antibodies.com Immunofluorescence - Anti-RASGRP1 Antibody (A8633) - Antibodies.com Immunofluorescence - Anti-RASGRP1 Antibody (A8633) - Antibodies.com Immunofluorescence - Anti-RASGRP1 Antibody (A8633) - Antibodies.com
$335
Product Datasheet

Rabbit polyclonal antibody to RASGRP1 for WB and ICC/IF.

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$40 to United States
Lead Time
5-8 Business Days

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Name
Anti-RASGRP1 Antibody
Description
Rabbit polyclonal antibody to RASGRP1.
Applications
WB, ICC/IF
Dilutions
WB: 1:500-1:1,000, ICC/IF: 1:50-1:200
Reactivity
Human, Mouse, Rat
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 598-797 of human RASGRP1 (NP_005730.2).
Sequence
PVAPTENNTSVGPVSNLCSLGAKDLLHAPEEGPFTFPNGEAVEHGEESKDRTIMLMGVSSQKISLRLKRAVAHKATQTESQPWIGSEGPSGPFVLSSPRKTAQDTLYVLPSPTSPCPSPVLVRKRAFVKWENKDSLIKSKEELRHLRLPTYQELEQEINTLKADNDALKIQLKYAQKKIESLQLEKSNHVLAQMEQGDCS
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugate

Unconjugated

Purification
Affinity purification.
Molecular Weight
90 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.02% Sodium Azide.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Synonyms
Calcium and DAG-regulated guanine nucleotide exchange factor II, CALDAG-GEFI, CalDAG-GEFII, GRP1_HUMAN, Guanine nucleotide exchange factor, calcium and DAG regulated, Rap1A, hRasGRP1, Ras activator RasGRP, RAS guanyl nucleotide releasing protein 1, RAS guanyl releasing protein 1, RAS guanyl releasing protein 1 (calcium and DAG-regulated), Ras guanyl-releasing protein, RAS guanyl-releasing protein 1, RASGRP, V
Isotype Controls
Suitable Secondaries
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
Western Blot - Anti-RASGRP1 Antibody (A8633) - Antibodies.comEnlarge Image
Figure 1: Western Blot - Anti-RASGRP1 Antibody (A8633)
Western blot analysis of extracts of THP-1 cells, using Anti-RASGRP1 Antibody (A8633) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 30s.
Immunofluorescence - Anti-RASGRP1 Antibody (A8633) - Antibodies.comEnlarge Image
Figure 2: Immunofluorescence - Anti-RASGRP1 Antibody (A8633)
Immunofluorescence analysis of A-549 cells using Anti-RASGRP1 Antibody (A8633) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).
Immunofluorescence - Anti-RASGRP1 Antibody (A8633) - Antibodies.comEnlarge Image
Figure 3: Immunofluorescence - Anti-RASGRP1 Antibody (A8633)
Immunofluorescence analysis of C6 cells using Anti-RASGRP1 Antibody (A8633) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).
Immunofluorescence - Anti-RASGRP1 Antibody (A8633) - Antibodies.comEnlarge Image
Figure 4: Immunofluorescence - Anti-RASGRP1 Antibody (A8633)
Immunofluorescence analysis of NIH/3T3 cells using Anti-RASGRP1 Antibody (A8633) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).
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