Rat Heat Shock 70kDa Protein 8 ELISA Kit (A6183)

Rat Heat Shock 70kDa Protein 8 ELISA Kit

Sandwich (quantitative)

The microtiter plate provided in this kit has been pre-coated with an antibody specific to the index. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to the index. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain the index, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of the index in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Microplate

Colorimetric

0.103 ng/ml

0.312 - 20 ng/ml

1 - 4.5 Hours

Rat

2410008N15Rik, Constitutive heat shock protein 70, Epididymis luminal protein 33, Epididymis secretory sperm binding protein Li 72p, Heat shock 70 kDa protein 8, Heat shock 70kD protein 10, Heat shock 70kD protein 8, Heat shock cognate 71 kDa protein, Heat shock cognate protein 54, Heat shock cognate protein 71 kDa, Heat shock protein 8, Heat shock protein A8, Heat shock protein family A (Hsp70) member 8, Heat-shock70-KD protein 10, formerly, HEL 33, HEL S 72p, HSC54, Hsc70, HSC71, Hsc73, HSP71, HSP73, HSP7C_HUMAN, HSPA10, HSPA8, LAP1, Lipopolysaccharide associated protein 1, LPS associated protein, LPS associated protein 1, MGC102007, MGC106514, MGC114311, MGC118485, MGC131511, MGC29929, N-myristoyltransferase inhibitor protein 71, NIP71