Rat IgG2a Isotype Control [RTG2A1-1] (PE) (A122149)

Rat IgG2a Isotype Control [RTG2A1-1] (PE) for Flow Cytometry.

Alternative Formats and ConjugatesAlternative Formats

Freight Charges
Lead Time
4-6 Business Days
Telephone
+1 (314) 370-6046
Mon - Fri, 8am - 4pm AST
Email
orders@antibodies.com
Name
Rat IgG2a Isotype Control [RTG2A1-1] (PE)
Specificity
This antibody, also known as clone G2A-1-1 or KLH/G2A-1-1, detects specifically the hemocyanin of keyhole limpet and can be used as an isotype control antibody in other species.
Applications
Flow Cytometry
Dilutions
Negative control: The reagent is intended as an isotype control to establish the amount of non-specific antibody binding. For your particular experiment, use the same concentration of this control antibody as the recommended working concentration of the antigen-specific antibody. Also, when working with prediluted antibodies, dilute the isotype control to the same concentration as is the concentration of the antigen-specific antibody in the prediluted antibody solution you are using. If under particular experimental conditions the background signal of the isotype control is too high (usually when working concentrations of used antibodies are above 10 µg/ml of incubation mixture), change the conditions of your experiment to reduce the background.
Immunogen
Keyhole limpet hemocyanin.
Host
Rat
Clonality
Monoclonal
Clone ID
RTG2A1-1
Isotype
IgG2a
Conjugate

PE

Excitation: 565nm, Emission: 578nm

Purification
This antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. The conjugate is purified by size-exclusion chromatography; removing unconjugated antibody and free fluorochrome.
Concentration
100 µg/ml
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.4, with 15 mM Sodium Azide.
Storage
Shipped at 4°C. Store in the dark at 2-8°C. Do not freeze!
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
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