Rat IL-1 beta ELISA Kit (High Sensitivity) (A323184)

$495
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Lead Time
7-10 Business Days
Telephone
+1 (314) 370-6046
Mon - Fri, 8am - 4pm AST
Email
orders@antibodies.com
Name
Rat IL-1 beta ELISA Kit (High Sensitivity)
Description
Rat IL-1 beta ELISA Kit is a high sensitivity sandwich Enzyme-Linked Immunosorbent Assay (sELISA) designed for the in vitro quantitative determination of rat IL-1 beta in serum, plasma, and other biological fluids.
Assay Type
Sandwich (quantitative)
Principle of Assay
Rat IL-1 beta ELISA Kit (A323184) employs the sandwich Enzyme-Linked Immunoassay technique for the quantitative measurement of rat IL-1 beta in serum, plasma, and other biological fluids. An antibody specific for IL-1 beta has been pre-coated onto a 96-well microtiter plate. The standards and test samples are added into the wells and the IL-1 beta present in each sample is bound to the wells by the immobilized antibody. Following incubation, the wells are washed and then incubated with Biotinylated Anti-IL-1 beta Antibody, which binds the captured IL-1 beta present in each well. Following incubation, unbound biotinylated detection antibody is removed by washing, and an HRP-Avidin conjugate is added to the wells and the microtiter plate is incubated. Following incubation and washing, TMB substrate solution is then used to visualize the HRP enzymatic reaction by catalysis to produce a blue-coloured product that changes to yellow after addition of acidic stop solution. The density of yellow is proportional to the amount of IL-1 beta captured in each well. The concentration of IL-1 beta can then be calculated by reading the O.D. absorbance at 450nm in a microplate reader and referring to the standard curve.
Platform
Pre-coated Microplate (12 x 8 well strips)
Detection Type
Colormetric
Instrument
Colorimetric Microplate Reader
Sample Type
Serum, plasma, and other biological fluids.
Sensitivity
0.2 pg/ml
Product Range
0.31-20 pg/ml
Assay Time
3h 30m
Reactivity
Rat
Cross Reactivity
No significant cross-reactivity or interference between rat IL-1 beta and analogues was observed.
Recovery
Sample Type n Range Average
Serum 8 95% - 110% 102%
EDTA Plasma 8 84% - 98% 90%
Cell Culture Media 8 87% - 99% 92%
Linearity
Sample Type 1:2 1:4 1:8 1:16
Serum (n=5) 86% - 97% 84% - 95% 86% - 101% 87% - 97%
EDTA Plasma (n=5) 84% - 95% 80% - 91% 80% - 92% 83% - 93%
Cell Culture Media (n=5) 93% - 107% 93% - 110% 95% - 111% 91% - 106%
Precision
Intra-assay CV: < 10%, Inter-assay CV: < 10%
Storage
Shipped at +4°C. Store short term at +4°C and long term at +4°C/-20°C. Refer to Protocol Manual. Avoid freeze/thaw cycles.
Synonyms
Catabolin, H1, Hematopoietin 1 , IFN beta inducing factor , IL 1 , IL 1 beta , IL 1B, IL 1beta, IL1 , IL1 beta, IL1B, IL1B_HUMAN, IL1F2 , Interleukin 1 beta , Interleukin 1 beta precursor , Interleukin-1 beta, LAF, OAF, Osteoclast activating factor , OTTHUMP00000162031, Preinterleukin 1 beta, Preinterleukin beta , Pro interleukin 1 beta
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
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