Rat NGAL ELISA Kit (A674)

Rat NGAL ELISA Kit

Sandwich (quantitative)

This NGAL enzyme linked immunosorbent assay (ELISA) applies a technique called a quantitative sandwich immunoassay. The microtiter plate has been pre-coated with an Anti-NGAL Antibody. Standards or samples are then added to the microtire plate wells and NGAL, if present, will bind to the antibody pre-coated on the wells. In order to quantify the amount of NGAL present in the sample, Anti-NGAL Antibody (HRP) is added to each well to "sandwich" the NGAL immobilised on the plate. The microtiter plate then undergoes incubation, followed by thorough washing of the wells to remove all unbound components. Next, a TMB (3,3',5,5' tetramethyl-benzidine) substrate solution is added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain NGAL will exhibit a change in colour. The enzyme-substrate reaction is terminated by the addition of a stop solution and the colour change is measured spectrophotometrically at a wavelength of 450nm ± 2nm.

Microplate

Serum, plasma, urine, cell culture supernatants, and tissue samples.

156-10,000 pg/ml

Rat

Store at 2-8°C for 6 months.

24p3, 25 kDa alpha 2 microglobulin related subunit of MMP9, 25 kDa alpha-2-microglobulin-related subunit of MMP-9, Alpha 2 microglobulin related protein, HGNC:6526, HNL, Lcn 2, Lcn2, Lipocalin 2, Lipocalin-2, Lipocalin-2 / NGAL, Migration stimulating factor inhibitor, MSFI, Neutrophil gelatinase associated lipocalin, Neutrophil gelatinase associated lipocalin precursor, Neutrophil gelatinase-associated lipocalin, NGAL_HUMAN, Oncogene 24p3, Oncogenic lipocalin 24p3, p25, Siderocalin, siderocalin LCN2, SV40 induced 24P3 protein, Uterocalin