Rat Periostin ELISA Kit (A7079)

Rat Periostin ELISA Kit

Sandwich (quantitative)

The microtiter plate provided in this kit has been pre-coated with an antibody specific to the index. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to the index. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain the index, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of the index in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Microplate

Colorimetric

43 pg/ml

125 - 8000 pg/ml

1 - 4.5 Hours

Rat

Fasciclin-I like, MGC119510, MGC119511, OSF 2, OSF-2, OSF2, Osteoblast specific factor, Osteoblast specific factor 2, Osteoblast specific factor 2 (fasciclin I like), Osteoblast-specific factor 2, PDLPOSTN, Periodontal ligament specific periostin, Periostin isoform thy2, Periostin isoform thy4, Periostin isoform thy6, Periostin isoform thy8, Periostin osteoblast specific factor, PN, POSTN, POSTN_HUMAN, RP11 412K4.1