Rat Peroxisome Proliferator Activated Receptor Gamma Coactivator 1 Alpha ELISA Kit (A5349)

Rat Peroxisome Proliferator Activated Receptor Gamma Coactivator 1 Alpha ELISA Kit

Sandwich (quantitative)

The microtiter plate provided in this kit has been pre-coated with an antibody specific to the index. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to the index. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain the index, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of the index in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Microplate

Colorimetric

Tissue homogenates, cell lysates and other biological fluids.

0.156 - 10 ng/ml

1 - 4.5 Hours

Rat

L PGC 1alpha, LEM6, Ligand effect modulator 6, Peroxisome proliferative activated receptor gamma coactivator 1, Peroxisome proliferative activated receptor gamma coactivator 1 alpha, Peroxisome proliferator activated receptor gamma coactivator 1 alpha transcript variant B4, Peroxisome proliferator activated receptor gamma coactivator 1 alpha transcript variant B4 3ext, Peroxisome proliferator activated receptor gamma coactivator 1 alpha transcript variant B4 8a, Peroxisome proliferator activated receptor gamma coactivator 1 alpha transcript variant B5, Peroxisome proliferator activated receptor gamma coactivator 1 alpha transcript variant B5 NT, Peroxisome proliferator-activated receptor gamma coactivator 1-alpha, PGC 1 (alpha), PGC 1 alpha, PGC 1v, PGC-1-alpha, PGC1, PGC1 alpha, PGC1(alpha), PGC1A, PGC1v, PPAR gamma coactivator 1, PPAR gamma coactivator 1 alpha, PPAR gamma coactivator 1 alpha 3 ligand effect modulator 6, PPAR gamma coactivator variant form, PPAR-gamma coactivator 1-alpha, PPARGC 1 alpha, PPARGC-1-alpha, PPARGC1, PPARGC1A, PRGC1_HUMAN