Rat TGF beta 1 ELISA Kit (A709)

Rat TGF beta 1 ELISA Kit

Sandwich (quantitative)

This TGF beta 1 enzyme linked immunosorbent assay (ELISA) applies a technique called a quantitative sandwich immunoassay. The microtiter plate has been pre-coated with an Anti-TGF beta 1 Antibody. Standards or samples are then added to the microtire plate wells and TGF beta 1, if present, will bind to the antibody pre-coated on the wells. In order to quantify the amount of TGF beta 1 present in the sample, Anti-TGF beta 1 Antibody (Biotin) is added to each well to "sandwich" the TGF beta 1 immobilised on the plate. The microtiter plate then undergoes incubation, followed by thorough washing of the wells to remove all unbound components. Next, Avidin-Biotin-Peroxidase Complex is added to each well for a short incubation period, followed by thorough washing of the wells to remove all unbound conjugates. Next, a TMB (3,3',5,5' tetramethyl-benzidine) substrate solution is added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain TGF beta 1 will exhibit a change in colour. The enzyme-substrate reaction is terminated by the addition of a stop solution and the colour change is measured spectrophotometrically at a wavelength of 450nm ± 2nm.

Microplate

Serum, body fluids, tissue lysates, and cell culture supernatants.

< 1 pg/ml

15.6-1000 pg/ml

Rat

Store at 2-8°C for 6 months or at -20°C for 12 months.

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