Rat VB 12 ELISA Kit (A79832)

This product is discontinued

Rat VB 12 ELISA Kit (A79832) has been discontinued and is no longer available.

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Name
Rat VB 12 ELISA Kit
Assay Type
Competitive (quantitative)
Principle of Assay
This Rat VB 12 ELISA Kit is an in vitro competitive enzyme-linked immunosorbent assay for the quantitative measurement of VB 12.

A microplate has been precoated with VB 12. Samples, standards and Anti-VB 12 Antibody (Biotin) are added to the wells, where VB 12 in the sample competes with the fixed VB 12 to bind to the antibody. The wells are washed to remove unbound material and HRP-conjugated streptavidin is added. After incubation, the wells are washed again and TMB substrate is added. The TMB substrate is catalyzed by HRP to produce blue colouration. The reaction is terminated by addition of Stop Solution which stops the colour development and produces a colour change from blue to yellow. The intensity of the signal is inversely proportional to the amount of VB 12 in the sample and the intensity is measured at 45 nm.
Platform
Pre-coated Microplate (12 x 8 well strips)
Detection Type
Colorimetric
Instrument
Colorimetric Microplate Reader
Sample Type
Serum, plasma, tissue homogenates, and other biological fluids.
Sensitivity
0.469 ng/ml
Product Range
0.781-50 ng/ml
Assay Time
4h 30m
Reactivity
Rat
Precision
Intra-assay CV: < 8%, Inter-assay CV: < 10%
General Notes
Information online is representative. Refer to the Instructions for Use inside the kit.
Storage
Store at +4°C. Do not use past expiration date!
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
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