Anti-Rb (phospho Ser780) Antibody [ARC0096] (A308931)

Name: Anti-Rb (phospho Ser780) Antibody [ARC0096]
Brand: Antibodies.com
SKU: A308931
Price: 540.0 USD
Availability: InStock

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$540

Rabbit monoclonal [ARC0096] antibody to Rb (phospho Ser780) for WB and IHC.

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Delivery

Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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Telephone

+1 (314) 370-6046

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Specifications

Name

Anti-Rb (phospho Ser780) Antibody [ARC0096]

Description

Rabbit monoclonal [ARC0096] antibody to Rb (phospho Ser780).

Applications

WB, IHC

Dilutions

WB: 1:500-1:2,000, IHC: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

A synthetic phosphorylated peptide around S780 of human RB (P06400).

Sequence

TLSPI

Host

Rabbit

Clonality

Monoclonal

Clone ID

ARC0096

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

106 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.05% Proclin 300.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

Exon 17 tumor GOS561 substitution mutation causes premature stop, GOS563 exon 17 substitution mutation causes premature stop, OSRC, Osteosarcoma, p105-Rb, P105RB, PP105, pp110, PPP1R130, pRb, Prepro retinoblastoma associated protein, Protein phosphatase 1 regulatory subunit 130, RB transcriptional corepressor 1, RB1, RB1 gene, RB_HUMAN, Retinoblastoma 1, Retinoblastoma suspectibility protein, Retinoblastoma-associated protein

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (4)

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Figure 1: Western Blot - Anti-Rb (phospho Ser780) Antibody [ARC0096] (A308931)

Western blot analysis of extracts of C6 cells, using Anti-Rb (phospho Ser780) Antibody [ARC0096] (A308931) at 1:1,000 dilution. C6 cells were treated by 10% FBS at 37°C for 30 minutes after serum-starvation overnightThe secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 10s.

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Figure 2: Immunohistochemistry - Anti-Rb (phospho Ser780) Antibody [ARC0096] (A308931)

Immunohistochemistry analysis of paraffin-embedded rat pancreas using Anti-Rb (phospho Ser780) Antibody [ARC0096] (A308931) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

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Figure 3: Immunohistochemistry - Anti-Rb (phospho Ser780) Antibody [ARC0096] (A308931)

Immunohistochemistry analysis of paraffin-embedded human colon tissue using Anti-Rb (phospho Ser780) Antibody [ARC0096] (A308931) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

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Figure 4: Immunohistochemistry - Anti-Rb (phospho Ser780) Antibody [ARC0096] (A308931)

Immunohistochemistry analysis of paraffin-embedded mouse liver using Anti-Rb (phospho Ser780) Antibody [ARC0096] (A308931) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

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