Anti-RbBP5 Antibody (A295267)

Name: Anti-RbBP5 Antibody
Brand: Antibodies.com
SKU: A295267
Price: 475.0 USD
Availability: InStock

Enlarge Image

$475

Rabbit polyclonal antibody to RbBP5 for WB, IP, IHC, ICC, ChIP, ChIP-chip and ChIP-Seq.

100% Guarantee

Price Match Guarantee

Delivery

Freight Charges

$40 to United States

Lead Time

5-8 Business Days

Contact Us

Telephone

+1 (314) 370-6046

orders@antibodies.com

Guarantees

100% Quality Guarantee

Price Match Guarantee

Specifications

Name

Anti-RbBP5 Antibody

Description

Rabbit polyclonal antibody to RbBP5.

Applications

WB, IP, IHC, ICC, ChIP, ChIP-chip, ChIP-Seq

Dilutions

ChIP: 1-3 µg, ChIP-chip: 10 µg, ChIP-Seq: 2 µg, ICC: 1:250-1:1,000, IHC: 1:1,000-1:5,000, IP: 2-10 µg / 1 mg lysate, WB: 1:10,000-1:25,000

Reactivity

Human

Immunogen

Synthetic peptide within amino acids 500 and the C-terminus of human RbBP5 (NP_005048. 2).

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Antigen affinity purification.

Concentration

1 mg/ml

Product Form

Liquid

Formulation

Supplied in Tris-Citrate/Phosphate Buffer, pH 7-8, with 0.09% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

General Notes

This antibody was affinity purified using the immunising peptide immobilized on solid support. Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1. 4 equals 1.0 mg of IgG.

Synonyms

RBBP 5, RBBP-5, RBBP5_HUMAN, RBQ 3, RBQ3, Retinoblastoma binding protein 5, Retinoblastoma binding protein RBQ3, Retinoblastoma binding protein5, Retinoblastoma-binding protein 5, Retinoblastoma-binding protein RBQ-3, SWD1, SWD1, Set1c WD40 repeat protein, homolog

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (5)

Enlarge Image

Figure 1: Western Blot - Anti-RbBP5 Antibody (A295267)

Samples: Whole cell lysate (50 µg) from HeLa, HEK293T, Jurkat, NCI-H460, and Malme-3M cells prepared using NETN lysis buffer. Antibody: Anti-RbBP5 Antibody (A295267) was used for WB at 0.04 µg/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.

Enlarge Image

Figure 2: Immunoprecipitation - Anti-RbBP5 Antibody (A295267)

Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Anti-RbBP5 Antibody (A295267) was used for IP at 6 µg per reaction. RbBP5 was also immunoprecipitated by a previous lot of this antibody. For blotting immunoprecipitated RbBP5, Anti-RbBP5 Antibody (A295267) was used at 0.04 µg/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.

Enlarge Image

Figure 3: Immunohistochemistry - Anti-RbBP5 Antibody (A295267)

Sample: FFPE section of human prostate carcinoma. Antibody: Anti-RbBP5 Antibody (A295267) was used at a dilution of 1:5,000 (0.2 µg/ml). Detection: DAB.

Enlarge Image

Figure 4: Anti-RbBP5 Antibody (A295267)

Chromatin from K562 cells was immunoprecipitated with Anti-RbBP5 Antibody (A295267) and analyzed by DNA sequencing. The figure illustrates the peak distribution of RbBP5 binding within a 500 Kb region of chromosome 1 as detected using Anti-RbBP5 Antibody (A295267). ChIP-seq validation performed by Diagenode, Denville, NJ.