Anti-RBBP7 Antibody [ARC55384] (A307025)

Name: Anti-RBBP7 Antibody [ARC55384]
Brand: Antibodies.com
SKU: A307025

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This product is discontinued

Anti-RBBP7 Antibody [ARC55384] (A307025) has been discontinued and is no longer available.

View all Anti-RBBP7 Antibodies.

Specifications

Name

Anti-RBBP7 Antibody [ARC55384]

Description

Rabbit monoclonal [ARC55384] antibody to RBBP7.

Applications

WB, IHC, ICC/IF

Dilutions

WB: 1:2,000-1:20,000, IHC: 1:100-1:500, ICC/IF: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-230 of human RbAp46/RBBP7 (NP_002884.1).

Sequence

MASKEMFEDTVEERVINEEYKIWKKNTPFLYDLVMTHALQWPSLTVQWLPEVTKPEGKDYALHWLVLGTHTSDEQNHLVVARVHIPNDDAQFDASHCDSDKGEFGGFGSVTGKIECEIKINHEGEVNRARYMPQNPHIIATKTPSSDVLVFDYTKHPAKPDPSGECNPDLRLRGHQKEGYGLSWNSNLSGHLLSASDDHTVCLWDINAGPKEGKIVDAKAIFTGHSAVVE

Host

Rabbit

Clonality

Monoclonal

Clone ID

ARC55384

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

50 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.05% Proclin 300.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

G1/S transition control protein binding protein RbAp46, Histone acetyltransferase type B subunit 2, Histone binding protein RBBP7, Histone-binding protein RBBP7, MGC138867, MGC138868, Nucleosome remodeling factor subunit RBAP46, Nucleosome-remodeling factor subunit RBAP46, RbAp46, RBBP 7, RBBP-7, RBBP7_HUMAN, Retinoblastoma binding protein 7, Retinoblastoma binding protein p46, Retinoblastoma-binding protein 7, Retinoblastoma-binding protein p46, Retinoblastoma-binding protein RbAp46

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (5)

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Figure 1: Western Blot - Anti-RBBP7 Antibody [ARC55384] (A307025)

Western blot analysis of various lysates, using Anti-RBBP7 Antibody [ARC55384] (A307025) at 1:20,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 20s.

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Figure 2: Immunohistochemistry - Anti-RBBP7 Antibody [ARC55384] (A307025)

Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue using Anti-RBBP7 Antibody [ARC55384] (A307025) at a dilution of 1:300(40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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Figure 3: Immunohistochemistry - Anti-RBBP7 Antibody [ARC55384] (A307025)

Immunohistochemistry analysis of paraffin-embedded rat kidney using Anti-RBBP7 Antibody [ARC55384] (A307025) at a dilution of 1:300(40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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Figure 4: Immunofluorescence - Anti-RBBP7 Antibody [ARC55384] (A307025)

Immunofluorescence analysis of NIH/3T3 using Anti-RBBP7 Antibody [ARC55384] (A307025) at a dilution of 1:100(40x lens). DAPI was used to stain the cell nuclei (blue).