Anti-RBCK1 Antibody (A308832)

Name: Anti-RBCK1 Antibody
Brand: Antibodies.com
SKU: A308832
Price: 270.0 GBP
Availability: InStock

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$355

Rabbit polyclonal antibody to RBCK1 for WB and ICC/IF.

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Shipping Information

Freight/Packing Charges: $40

Dispatched from St. Louis, MO.

Lead Time: 7-10 business days.

Specifications

Name

Anti-RBCK1 Antibody

Description

Rabbit polyclonal antibody to RBCK1.

Applications

WB, ICC/IF

Dilutions

WB: 1:500-1:1,000, ICC/IF: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 60-192 of human RBCK1 (NP_112506.2).

Sequence

SVEDAQMHTVTIWLTVRPDMTVASLKDMVFLDYGFPPVLQQWVIGQRLARDQETLHSHGVRQNGDSAYLYLLSARNTSLNPQELQRERQLRMLEDLGFKDLTLQPRGPLEPGPPKPGVPQEPGRGQPDAVPEP

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Concentration

Lot Specific

Molecular Weight

58 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.05% Proclin 300.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.

Synonyms

C20orf18, HBV-associated factor 4, Heme-oxidized IRP2 ubiquitin ligase 1, Hepatitis B virus X-associated protein 4, HOIL-1, RanBP-type and C3HC4-type zinc finger-containing protein 1, RING finger protein 54, RING-type E3 ubiquitin transferase HOIL-1, RNF54, UBCE7IP3, Ubiquitin-conjugating enzyme 7-interacting protein 3, XAP3, XAP4

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (4)

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Western Blot - Anti-RBCK1 Antibody (A308832)

Western blot analysis of extracts of various cell lines, using Anti-RBCK1 Antibody (A308832) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 90s.

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Immunofluorescence - Anti-RBCK1 Antibody (A308832)

Immunofluorescence analysis of U2OS cells using Anti-RBCK1 Antibody (A308832) at a dilution of 1:100. DAPI was used to stain the cell nuclei (blue).

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Immunofluorescence - Anti-RBCK1 Antibody (A308832)

Immunofluorescence analysis of NIH/3T3 cells using Anti-RBCK1 Antibody (A308832) at a dilution of 1:100. DAPI was used to stain the cell nuclei (blue).