Anti-REA Antibody [ARC1449] (A308350)

Name: Anti-REA Antibody [ARC1449]
Brand: Antibodies.com
SKU: A308350
Price: 470.0 USD
Availability: InStock

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$470

Rabbit monoclonal [ARC1449] antibody to REA for WB, IHC, ICC/IF and IP.

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Delivery

Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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+1 (314) 370-6046

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Specifications

Name

Anti-REA Antibody [ARC1449]

Description

Rabbit monoclonal [ARC1449] antibody to REA.

Applications

WB, IHC, ICC/IF, IP

Dilutions

WB: 1:500-1:2,000, IHC: 1:50-1:200, ICC/IF: 1:50-1:200, IP: 1:500-1:1,000

Reactivity

Human, Mouse, Rat

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 100-200 of human Prohibitin 2 (Prohibitin 2 (PHB2)) (Q99623).

Sequence

QMVNISLRVLSRPNAQELPSMYQRLGLDYEERVLPSIVNEVLKSVVAKFNASQLITQRAQVSLLIRRELTERAKDFSLILDDVAITELSFSREYTAAVEAK

Host

Rabbit

Clonality

Monoclonal

Clone ID

ARC1449

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

33 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

B cell receptor associated protein BAP37, B-cell receptor-associated protein BAP37, BAP, Bap37, BCAP 37, D prohibitin, D-prohibitin, p22, Phb2, PHB2_HUMAN, PNAS 141, Prohibitin 2, Prohibitin-2, Repressor of estrogen receptor activity

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (5)

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Figure 1: Western Blot - Anti-REA Antibody [ARC1449] (A308350)

Western blot analysis of extracts of various cell lines, using Anti-REA Antibody [ARC1449] (A308350) at 1:5,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 1s.

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Figure 2: Immunohistochemistry - Anti-REA Antibody [ARC1449] (A308350)

Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue using Anti-REA Antibody [ARC1449] (A308350) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

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Figure 3: Immunofluorescence - Anti-REA Antibody [ARC1449] (A308350)

Immunofluorescence analysis of NIH-3T3 cells using Anti-REA Antibody [ARC1449] (A308350) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).

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Figure 4: Immunofluorescence - Anti-REA Antibody [ARC1449] (A308350)

Immunofluorescence analysis of U-2 OS cells using Anti-REA Antibody [ARC1449] (A308350) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).