The protein has a predicted molecular mass of 36.1 kDa after removal of the signal peptide. The apparent molecular mass of 5T4-His is approximately 55-70 kDa due to glycosylation.
Conjugate
Unconjugated
Purity
> 95%, by SDS-PAGE and Coomassie blue staining.
Product Form
Lyophilized
Concentration
Reconstitution dependent.
Formulation
Lyophilized from sterile Phosphate Buffered Saline, pH 7.4. Normally 5%–8% Trehalose is added as a protectant before lyophilization.
Storage
Shipped at 4°C. Lyophilized: Store at -20°C to -80°C. Reconstituted: Aliquot and store at -80°C. Product is stable for one year. Avoid freeze/thaw cycles.
Figure 2: Recombinant Human 5T4 Protein (6xHis Tag) (A318304)
ELISA plates were pre-coated with Recombinant Human 5T4 Protein (6×His Tag) (A318304) at 1 µg/ml (100 µl/well) which can bind Anti-5T4 Rabbit mAb in a linear range of 3.2-16 ng/ml.
Figure 3: Recombinant Human 5T4 Protein (6xHis Tag) (A318304)
ELISA plates were pre-coated with Recombinant Human 5T4 Protein (6×His Tag) (A318304) at 2 µg/ml (100 µl/well) which can bind H8 Biosimilar - Anti-5T4 Antibody - BSA and Azide free (A318817) in a linear range of 0.64-16 ng/ml.
Figure 4: Recombinant Human 5T4 Protein (6xHis Tag) (A318304)
ELISA plates were pre-coated with Recombinant Human 5T4 Protein (6×His Tag) (A318304) at 2 µg/ml (100 µl/well) which can bind GEN-1044 Biosimilar - Anti-5T4 Antibody - BSA and Azide free (A318816) in a linear range of 0.64-16 ng/ml.
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